Developmental shift to a mechanism of synaptic vesicle endocytosis requiring nanodomain Ca2+

Takayuki Yamashita, Kohgaku Eguchi, Naoto Saitoh, Henrique von Gersdorff, Tomoyuki Takahashi

研究成果: Article査読

68 被引用数 (Scopus)

抄録

Ca2+ is thought to be essential for the exocytosis and endocytosis of synaptic vesicles. However, the manner in which Ca2+ coordinates these processes remains unclear, particularly at mature synapses. Using membrane capacitance measurements from calyx of Held nerve terminals in rats, we found that vesicle endocytosis is initiated primarily in Ca 2+ nanodomains around Ca2+ channels, where exocytosis is triggered. Bulk Ca2+ outside of the domain could also be involved in endocytosis at immature synapses, although only after extensive exocytosis at more mature synapses. This bulk Ca2+-dependent endocytosis required calmodulin and calcineurin activation at immature synapses, but not at more mature synapses. Similarly, GTP-independent endocytosis, which occurred after extensive exocytosis at immature synapses, became negligible after maturation. We propose that nanodomain Ca2+ simultaneously triggers exocytosis and endocytosis of synaptic vesicles and that the molecular mechanisms underlying Ca 2+-dependent endocytosis undergo major developmental changes at this fast central synapse.

本文言語English
ページ(範囲)838-844
ページ数7
ジャーナルNature Neuroscience
13
7
DOI
出版ステータスPublished - 07-2010
外部発表はい

All Science Journal Classification (ASJC) codes

  • 神経科学(全般)

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