Dexmedetomidine preserves the endothelial glycocalyx and improves survival in a rat heatstroke model

Kensuke Kobayashi, Soichiro Mimuro, Tsunehisa Sato, Atsushi Kobayashi, Shingo Kawashima, Hiroshi Makino, Matsuyuki Doi, Takasumi Katoh, Yoshiki Nakajima

研究成果: Article査読

3 被引用数 (Scopus)

抄録

Purpose: Heatstroke causes systemic inflammation, followed by vascular endothelial damage. The normal vascular endothelium is coated by endothelial glycocalyx (EGCX). Dexmedetomidine (DEX) has an anti-inflammatory effect, but there has been little investigation on the influence of heatstroke on EGCX and the effect of DEX on this condition. Therefore, we examined whether EGCX was disrupted in heatstroke and if DEX improved survival and preserves EGCX. Methods: Anesthetized Wistar rats were randomly assigned to three groups: a DEX group treated with DEX (5 µg/kg/h) and 0.9% saline infused continuously at 10 ml/kg/h during heat exposure; a NSS group given 0.9% saline during heat exposure; and a SHAM group given 0.9% saline alone without heat exposure. Heatstroke was induced by exposure to an ambient temperature of 40 °C with relative humidity of 60%. The survival rate was assessed up to 2 h after the start of heat exposure. Plasma levels of syndecan-1 and the thickness of EGCX using electron microscopy were measured when the systolic blood pressure fell to less than 80 mmHg. Results: The survival rate after 2 h of heat exposure was significantly higher in the DEX group compared to the NSS group (89% vs. 22%, P = 0.004). Plasma levels of syndecan-1 were 0.6 ± 1.3, 9.7 ± 5.9, and 2.1 ± 3.4 ng/ml in the SHAM, NSS and DEX groups, respectively (P = 0.013). The thickness of EGCX was significantly higher in the DEX group compared with the NSS group (P = 0.001). Conclusions: EGCX was disrupted in heatstroke, and DEX improved survival and preserved EGCX.

本文言語English
ページ(範囲)880-885
ページ数6
ジャーナルJournal of Anesthesia
32
6
DOI
出版ステータスPublished - 01-12-2018
外部発表はい

All Science Journal Classification (ASJC) codes

  • Anesthesiology and Pain Medicine

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