DGKζ is involved in LPS-activated phagocytosis through IQGAP1/Rac1 pathway

Masashi Okada, Yasukazu Hozumi, Kiyoshi Iwazaki, Kentaro Misaki, Mitsuaki Yanagida, Yoshihiko Araki, Takashi Watanabe, Hitoshi Yagisawa, Matthew K. Topham, Kozo Kaibuchi, Kaoru Goto

研究成果: Article査読

14 被引用数 (Scopus)

抄録

Diacylglycerol kinase (DGK) plays an important role in phosphoinositide signaling cascade by regulating the intracellular level of diacylglycerol and phosphatidic acid. The DGK family is involved in various pathophysiological responses that are mediated through unique binding partners in different tissues and cells. In this study, we identified a small GTPase effector protein, IQGAP1, as a novel DGKζ-associated complex protein. A bacterial endotoxin, lipopolysaccharide (LPS), facilitated the complex formation in macrophages. Both proteins co-localized at the edge and phagocytic cup of the cell. Furthermore, RNA interference-mediated knockdown of DGKζ or IQGAP1 impaired LPS-induced Rac1 activation. Primary macrophages derived from DGKζ-/- mice attenuated LPS-induced phagocytosis of bacteria. These results suggest that DGKζ is involved in IQGAP1/Rac1-mediated phagocytosis upon LPS stimulation in macrophages.

本文言語English
ページ(範囲)479-484
ページ数6
ジャーナルBiochemical and Biophysical Research Communications
420
2
DOI
出版ステータスPublished - 06-04-2012
外部発表はい

All Science Journal Classification (ASJC) codes

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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