Diagnostic test property of transcription-reverse transcription concerted reaction reagent TRCReady® SARS-CoV-2 i using nasopharyngeal swab samples

Megumi Ogawa, Manami Sato, Asuka Yamakami, Yuki Hayasaka, Yoshiko Saya, Kurando Une, Satoshi Iwai, Shigeo Tsuchiya, Yumiko Mikami, Chizumi Fukazawa, Yuki Uehara

研究成果: ジャーナルへの寄稿学術論文査読

抄録

TRCReady® SARS-CoV-2 i is a reagent for transcription-reverse transcription concerted reaction (TRC) to detect SARS-CoV-2 N2 gene, used with the automated rapid isothermal nucleic acid amplification test (NAAT) analyzer TRCReady®-80. Sensitivity and specificity of TRCReady® SARS-CoV-2 i was assessed by comparison with the results of real-time reverse transcription-polymerase chain reaction (RT-PCR) using nasopharyngeal swab samples. From November 2020 to March 2021, a total of 441 nasopharyngeal swabs were obtained and analyzed both with TRCReady® SARS-CoV-2 i and RT-PCR. Sensitivity and specificity of TRCReady® SARS-CoV-2 i were 94.6% (53/56) and 99.2% (382/385), respectively. Reaction time to positivity of TRCReady® SARS-CoV-2 i ranged from 1.166 to 9.805 (median: 2.887) min, and minimum detection sensitivity of TRCReady® SARS-CoV-2 i was 9 copies per test, with reaction time as 5.014 min. Detection of SARS-CoV-2 gene from nasopharyngeal swab sample using TRCReady® SARS-CoV-2 i shows comparative diagnostic test accuracy with RT-PCR, and can be used as a useful test to diagnose SARS-CoV-2 infection.

本文言語英語
ページ(範囲)115-117
ページ数3
ジャーナルJournal of Infection and Chemotherapy
29
1
DOI
出版ステータス出版済み - 01-2023

All Science Journal Classification (ASJC) codes

  • 微生物学(医療)
  • 感染症
  • 薬理学(医学)

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