TY - JOUR
T1 - Differences in MHC class I genes between strains of rainbow trout (Oncorhynchus mykiss)
AU - Xia, Chun
AU - Kiryu, Ikunari
AU - Dijkstra, Johannes Martinus
AU - Azuma, Teruo
AU - Nakanishi, Teruyuki
AU - Ototake, Mitsuru
N1 - Funding Information:
This study was supported by ‘the promotion of basic research activities for innovative biosciences’ funded by Bio-oriented Technology Research Advancement Institution (BRAIN), Japan, and by postdoctoral fellowships for C. Xia and J. M. Dijkstra from the Science and Technology Agency of Japan. We thank Dr James D. Moore, Bodega Marine Laboratory, U.S.A. for his help to edit this manuscript.
PY - 2002
Y1 - 2002
N2 - In rainbow trout there is only one dominant classical MHC class I locus, Onmy-UBA, for which four very different allelic lineages have been described. The purpose of the present study was to determine if Onmy-UBA polymorphism could be used for strain characterisation. This was performed by lineage-specific PCR investigation of 30 fish, each of the Nikko and Donaldson strains, and by sequence analysis of 25 of the amplified DNA fragments. Two new MHC class I lineages were detected in addition to the four previously described lineages, thus six distinct lineages were observed within the fish examined (Sal-MHCIa*A-F). The distribution of lineages appeared to be strain-specific. For example, the lineage Sal-MHCIa*A was very common in the Nikko strain but could not be detected in the Donaldson strain. Analysis of MHC class I variation may help to elucidate relationships between strains and the roles of MHC alleles in disease resistance.
AB - In rainbow trout there is only one dominant classical MHC class I locus, Onmy-UBA, for which four very different allelic lineages have been described. The purpose of the present study was to determine if Onmy-UBA polymorphism could be used for strain characterisation. This was performed by lineage-specific PCR investigation of 30 fish, each of the Nikko and Donaldson strains, and by sequence analysis of 25 of the amplified DNA fragments. Two new MHC class I lineages were detected in addition to the four previously described lineages, thus six distinct lineages were observed within the fish examined (Sal-MHCIa*A-F). The distribution of lineages appeared to be strain-specific. For example, the lineage Sal-MHCIa*A was very common in the Nikko strain but could not be detected in the Donaldson strain. Analysis of MHC class I variation may help to elucidate relationships between strains and the roles of MHC alleles in disease resistance.
UR - https://www.scopus.com/pages/publications/0036012803
UR - https://www.scopus.com/pages/publications/0036012803#tab=citedBy
U2 - 10.1006/fsim.2001.0371
DO - 10.1006/fsim.2001.0371
M3 - Article
C2 - 12049167
AN - SCOPUS:0036012803
SN - 1050-4648
VL - 12
SP - 287
EP - 301
JO - Fish and Shellfish Immunology
JF - Fish and Shellfish Immunology
IS - 4
ER -