Differences in the mechanism of nitric oxide production between mouse vascular endothelial cells and macrophages

Tsuyoshi Sugiyama, Megumi Fujita, Naoki Koide, Akiko Morikawa, Kazuko Takahashi, Tomoaki Yoshida, Hiroshi Mori, Takashi Yokochi

研究成果: Article査読

7 被引用数 (Scopus)

抄録

The detailed mechanism of NO production in mouse vascular endothelial cells, END-D, was studied. The NO production in END-D cells was triggered by gamma interferon (IFN-γ), but not LPS. However, LPS augmented the NO production in IFN-γ-stimulated END-D cells. A high level of NO production was due to the expression of an inducible type of NO synthase (iNOS) in those cells. A significant amount of NO was detected 18 h after IFN-γ stimulation, accompanied by the delayed iNOS expression. The JAK/STAT signal pathway mediated IFN-γ-induced NO production, but did not participate in the LPS-induced augmentation. Further, no activation of nuclear factor (NF -κB was involved in the NO production in END-D cells stimulated with either IFN-γ and/or LPS. The mechanism of NO production in END-D cells was suggested to be different from that in mouse macrophages. The differential regulation of NO production in mouse vascular endothelial cells and macrophages is discussed.

本文言語English
ページ(範囲)108-112
ページ数5
ジャーナルJournal of Endotoxin Research
9
2
DOI
出版ステータスPublished - 2003
外部発表はい

All Science Journal Classification (ASJC) codes

  • 微生物学
  • 免疫学
  • 毒物学

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