Differential modulation of surfactant protein D under acute and persistent hypoxia in acute lung injury

Koji Sakamoto, Naozumi Hashimoto, Yasuhiro Kondoh, Kazuyoshi Imaizumi, Daisuke Aoyama, Takashi Kohnoh, Masaaki Kusunose, Motohiro Kimura, Tsutomu Kawabe, Hiroyuki Taniguchi, Yoshinori Hasegawa

研究成果: Article査読

19 被引用数 (Scopus)

抄録

Hypoxia contributes to the development of fibrosis with epithelial-mesenchymal transition (EMT) via stimulation of hypoxia-inducible factor 1α (HIF-1α) and de novo twist expression. Although hypoxemia is associated with increasing levels of surfactant protein D (SP-D) in acute lung injury (ALI), the longitudinal effects of hypoxia on SP-D expression in lung tissue injury/fibrosis have not been fully evaluated. Here, the involvement of hypoxia and SP-D modulation was evaluated in a model of bleomycin- induced lung injury. We also investigated the molecular mechanisms by which hypoxia might modulate SP-D expression in alveolar cells, by using a doxycycline (Dox)-dependent HIF-1α expression system. Tissue hypoxia and altered SP-D levels were present in bleomycin-induced fibrotic lesions. Acute hypoxia induced SP-D expression, supported by the finding that Dox-induced expression of HIF-1α increased SP-D expression. In contrast, persistent hypoxia repressed SP-D expression coupled with an EMT phenotype and twist expression. Long-term expression of HIF-1α caused SP-D repression with twist expression. Ectopic twist expression repressed SP-D expression. The longitudinal observation of hypoxia and SP-D levels in ALI in vivo was supported by the finding that HIF-1α expression stabilized by acute hypoxia induced increasing SP-D expression in alveolar cells, whereas persistent hypoxia induced de novo twist expression in these cells, causing repression of SP-D and acquisition of an EMT phenotype. Thus this is the first study to demonstrate the molecular mechanisms, in which SP-D expression under acute and persistent hypoxia in acute lung injury might be differentially modulated by stabilized HIF-1α expression and de novo twist expression.

本文言語English
ページ(範囲)L43-L53
ジャーナルAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
303
1
DOI
出版ステータスPublished - 01-07-2012

All Science Journal Classification (ASJC) codes

  • 生理学
  • 呼吸器内科
  • 生理学(医学)
  • 細胞生物学

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