Direct detection of human herpesvirus 6 DNA in serum by variant specific loop-mediated isothermal amplification in hematopoietic stem cell transplant recipients

Masaru Ihira; Hiroko Sugiyama; Yoshihiko Enomoto; Yuki Higashimoto; Ken Sugata; Yoshizo Asano; Tetsushi Yoshikawa

doi:10.1016/j.jviromet.2010.01.025

Direct detection of human herpesvirus 6 DNA in serum by variant specific loop-mediated isothermal amplification in hematopoietic stem cell transplant recipients

Masaru Ihira
, Hiroko Sugiyama
, Yoshihiko Enomoto
, Yuki Higashimoto
, Ken Sugata
, Yoshizo Asano
, Tetsushi Yoshikawa

研究成果: ジャーナルへの寄稿 › 学術論文 › 査読

16 被引用数 (Scopus)

抄録

A variant specific direct loop-mediated isothermal amplification (LAMP) method was developed to detect human herpesvirus-6 (HHV-6) variants in serum samples. Specific primers were designed against HHV-6 U86 gene. Initial validation analysis confirmed high specificity and sensitivity of the method. This method was shown to be highly reliable for monitoring active HHV-6 infection in hematopoietic stem cell transplant recipients.

本文言語	英語
ページ（範囲）	103-106
ページ数	4
ジャーナル	Journal of Virological Methods
巻	167
号	1
DOI	https://doi.org/10.1016/j.jviromet.2010.01.025
出版ステータス	出版済み - 07-2010

All Science Journal Classification (ASJC) codes

ウイルス学

文献へのアクセス

10.1016/j.jviromet.2010.01.025

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引用スタイル

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abstract = "A variant specific direct loop-mediated isothermal amplification (LAMP) method was developed to detect human herpesvirus-6 (HHV-6) variants in serum samples. Specific primers were designed against HHV-6 U86 gene. Initial validation analysis confirmed high specificity and sensitivity of the method. This method was shown to be highly reliable for monitoring active HHV-6 infection in hematopoietic stem cell transplant recipients.",

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AU - Higashimoto, Yuki

AU - Sugata, Ken

AU - Asano, Yoshizo

AU - Yoshikawa, Tetsushi

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