TY - JOUR
T1 - Discovery, development, and testing of substrates and inhibitors of pp60c-SRC
AU - Budde, Raymond J.A.
AU - Mcmurray, John S.
AU - Saya, Hideyuki
AU - Gallick, Gary E.
AU - Levin, Victor A.
PY - 1995
Y1 - 1995
N2 - Currently, there are no specific protein tyrosine kinase inhibitors available. This review summarizes our efforts to develop an active-site-directed inhibitor of pp60c-srv Initial efforts are directed at determining substrate specificity with synthetic peptides and at developing a biological system to test the potential of pp60c-src inhibitors to effectively inhibit the growth of pp60c-src activated cell lines. To meet these goals, we have developed new methods for purifying recombinant pp60C-SRC, assaying tyrosine kinase activity, synthesizing cyclic peptides, and generating random peptide libraries. In addition, we discuss the generation of potential artifacts while using polyhydroxy aromatic compounds as tyrosine kinase inhibitors.
AB - Currently, there are no specific protein tyrosine kinase inhibitors available. This review summarizes our efforts to develop an active-site-directed inhibitor of pp60c-srv Initial efforts are directed at determining substrate specificity with synthetic peptides and at developing a biological system to test the potential of pp60c-src inhibitors to effectively inhibit the growth of pp60c-src activated cell lines. To meet these goals, we have developed new methods for purifying recombinant pp60C-SRC, assaying tyrosine kinase activity, synthesizing cyclic peptides, and generating random peptide libraries. In addition, we discuss the generation of potential artifacts while using polyhydroxy aromatic compounds as tyrosine kinase inhibitors.
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U2 - 10.3109/13880209509067085
DO - 10.3109/13880209509067085
M3 - Article
AN - SCOPUS:84907110765
SN - 1388-0209
VL - 33
SP - 27
EP - 34
JO - Pharmaceutical Biology
JF - Pharmaceutical Biology
IS - S1
ER -