TY - JOUR
T1 - Discovery of a novel type of autophagy targeting RNA
AU - Fujiwara, Yuuki
AU - Furuta, Akiko
AU - Kikuchi, Hisae
AU - Aizawa, Shu
AU - Hatanaka, Yusuke
AU - Konya, Chiho
AU - Uchida, Kenko
AU - Yoshimura, Aya
AU - Tamai, Yoshitaka
AU - Wada, Keiji
AU - Kabuta, Tomohiro
N1 - Funding Information:
We thank Yohei Fujimoto, Hiromi Fujita, Yoshiko Hara and Tomoko Okada for technical assistance, Drs. Yoshitaka Nagai, Hirohiko Hohjoh and Toshihide Takeuchi for helpful discussions, and Drs. Judith Blanz and Paul Saftig for the LAMP2-deficient mice. This work was supported by a Grant-in-Aid for Young Scientists (A) (24680038 to T.K.) and Grants-in-Aid for Scientific Research (to K.W.) from the Japan Society for the Promotion of Science; by the Program for Promotion of Fundamental Studies in Health Sciences of the National Institute of Biomedical Innovation (NIBIO), Japan (to K.W.); and partly by a Grant-in-Aid for Young Scientists (B) (22790838 to T.K.) and Grants-in-Aid for Scientific Research on Nervous and Mental Disorders from the Ministry of Health, Labour, and Welfare of Japan (21-9, 23-1 and 24-11 to T.K.).
PY - 2013/3
Y1 - 2013/3
N2 - Regulated degradation of cellular components by lysosomes is essential to maintain biological homeostasis. In mammals, three forms of autophagy, macroautophagy, microautophagy and chaperone-mediated autophagy (CMA), have been identified. Here, we showed a novel type of autophagy, in which RNA is taken up directly into lysosomes for degradation. This pathway, which we term 'RNautophagy,' is ATP-dependent, and unlike CMA, is independent of HSPA8/ Hsc70. LAMP2C, a lysosomal membrane protein, serves as a receptor for this pathway. The cytosolic tail of LAMP2C specifically binds to almost all total RNA derived from mouse brain. The cytosolic sequence of LAMP2C and its affinity for RNA are evolutionarily conserved from nematodes to humans. Our findings shed light on the mechanisms underlying RNA homeostasis in higher eukaryotes.
AB - Regulated degradation of cellular components by lysosomes is essential to maintain biological homeostasis. In mammals, three forms of autophagy, macroautophagy, microautophagy and chaperone-mediated autophagy (CMA), have been identified. Here, we showed a novel type of autophagy, in which RNA is taken up directly into lysosomes for degradation. This pathway, which we term 'RNautophagy,' is ATP-dependent, and unlike CMA, is independent of HSPA8/ Hsc70. LAMP2C, a lysosomal membrane protein, serves as a receptor for this pathway. The cytosolic tail of LAMP2C specifically binds to almost all total RNA derived from mouse brain. The cytosolic sequence of LAMP2C and its affinity for RNA are evolutionarily conserved from nematodes to humans. Our findings shed light on the mechanisms underlying RNA homeostasis in higher eukaryotes.
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U2 - 10.4161/auto.23002
DO - 10.4161/auto.23002
M3 - Article
C2 - 23291500
AN - SCOPUS:84876222448
SN - 1554-8627
VL - 9
SP - 403
EP - 409
JO - Autophagy
JF - Autophagy
IS - 3
ER -