Dominant expression of ATP-binding cassette transporter-1 on basolateral surface of Caco-2 cells stimulated by LXR/RXR ligands

Tohru Ohama, Ken ichi Hirano, Zhongyan Zhang, Ryo Aoki, Ken ichi Tsujii, Yumiko Nakagawa-Toyama, Kosuke Tsukamoto, Chiaki Ikegami, Akifumi Matsuyama, Masato Ishigami, Naohiko Sakai, Hisatoyo Hiraoka, Kazumitsu Ueda, Shizuya Yamashita, Yuji Matsuzawa

研究成果: Article

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ATP-binding cassette transporter-1 (ABCA1) is a cause of Tangier disease, which is a familial deficiency of plasma high density lipoproteins (HDL). This molecule is known to be expressed in the multiple tissues and organs including small intestines, liver, and macrophages in the blood vessels. Recent in vivo studies suggested that ABCA1 plays some roles in the flux of cholesterol in the intestines. One of the major questions to understand the roles of ABCA1 in the intestines is the expression pattern in the intestinal epithelial cells. To address this issue, we have investigated the expression and regulation of ABCA1 in Caco-2 cells cultured on Transwell as a model, especially focusing on possible polarized expression of ABCA1. The expression of ABCA1 was up-regulated during the differentiation and under the stimulation of LXR/RXR by the addition of 9-cis-retinoic acid (9-cis-RA) and 22-R-hydroxycholesterol (22-OH). Apolipoprotein-AI-mediated cholesterol efflux was dominant toward the basolateral side of polarized cells when stimulated by 9-cis-RA and 22-OH. The cell surface biotinylation experiment followed by Western blot analyses demonstrated a markedly dominant expression of ABCA1 on the basolateral surface, which was clearly confirmed by the confocal laser scanning microscopy. In conclusion, the present study demonstrates that ABCA1 is dominantly expressed on the basolateral surface of Caco-2 cells tested, suggesting that this molecule may play a role in the basolateral movement of cholesterol at least when stimulated by LXR/RXR ligands.

元の言語English
ページ(範囲)625-630
ページ数6
ジャーナルBiochemical and Biophysical Research Communications
296
発行部数3
DOI
出版物ステータスPublished - 2002

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Ohama, T., Hirano, K. I., Zhang, Z., Aoki, R., Tsujii, K. I., Nakagawa-Toyama, Y., Tsukamoto, K., Ikegami, C., Matsuyama, A., Ishigami, M., Sakai, N., Hiraoka, H., Ueda, K., Yamashita, S., & Matsuzawa, Y. (2002). Dominant expression of ATP-binding cassette transporter-1 on basolateral surface of Caco-2 cells stimulated by LXR/RXR ligands. Biochemical and Biophysical Research Communications, 296(3), 625-630. https://doi.org/10.1016/S0006-291X(02)00853-7