TY - JOUR
T1 - Down-regulation of endodermal Shh is required for gland formation in chicken stomach
AU - Fukuda, Kimiko
AU - Kameda, Takashi
AU - Saitoh, Kanako
AU - Iba, Hideo
AU - Yasugi, Sadao
N1 - Funding Information:
We thank Dr Nohno, Dr Kuroiwa, Dr Scotting and Dr Tabin for probes and Dr Gaffield for cyclopamine. We also thank Dr Stern and Dr Wakamatsu for their helpful comments on the manuscript. This work was supported in part by Grants-in-aid from the Ministry of Education, Technology, Science and Culture of Japan to K.F. and S.Y.
PY - 2003/7
Y1 - 2003/7
N2 - During the development of the proventriculus (glandular stomach) of the chicken embryo, the endodermal epithelium invades into the surrounding mesenchyme and forms glands. The glandular epithelial cells produce pepsinogen, while the non-glandular (luminal) epithelial cells secrete mucus. Sonic hedgehog is expressed uniformly in the proventricular epithelium before gland formation, but its expression ceases in gland cells. Here we present evidence that down-regulation of Sonic hedgehog is necessary for gland formation in the epithelium using a specific inhibitor of Sonic hedgehog signaling and virus mediated overexpression of Sonic hedgehog. We also show that gland formation is not induced by down-regulation of Sonic hedgehog alone; a mesenchymal influence is also required.
AB - During the development of the proventriculus (glandular stomach) of the chicken embryo, the endodermal epithelium invades into the surrounding mesenchyme and forms glands. The glandular epithelial cells produce pepsinogen, while the non-glandular (luminal) epithelial cells secrete mucus. Sonic hedgehog is expressed uniformly in the proventricular epithelium before gland formation, but its expression ceases in gland cells. Here we present evidence that down-regulation of Sonic hedgehog is necessary for gland formation in the epithelium using a specific inhibitor of Sonic hedgehog signaling and virus mediated overexpression of Sonic hedgehog. We also show that gland formation is not induced by down-regulation of Sonic hedgehog alone; a mesenchymal influence is also required.
UR - https://www.scopus.com/pages/publications/0042707883
UR - https://www.scopus.com/pages/publications/0042707883#tab=citedBy
U2 - 10.1016/S0925-4773(03)00069-8
DO - 10.1016/S0925-4773(03)00069-8
M3 - Article
C2 - 12915230
AN - SCOPUS:0042707883
SN - 0925-4773
VL - 120
SP - 801
EP - 809
JO - Mechanisms of Development
JF - Mechanisms of Development
IS - 7
ER -