E-selectin targeting to visualize tumors in vivo

Masahiko Hirai, Yoshie Hiramatsu, Shinki Iwashita, Takayuki Otani, Ling Chen, Yue Guang Li, Masashi Okada, Kazunori Oie, Koich Igarashi, Hideaki Wakita, Masaharu Seno

研究成果: Article

10 引用 (Scopus)

抜粋

Generally angiogenic factors induce the expression of E-selectin in vascular endothelial cells in the tumors. In this study, we employed an anti-E-selectin monoclonal antibody to target tumors in vivo and evaluated an optical imaging reagent to visualize tumor regions. The anti-E-selectin antibody was conjugated on the surface of liposomes, which encapsulated the near-infrared fluorescent substances Cy3 or Cy5.5. The liposomes efficiently recognized human umbilical vein endothelial cells only when E-selectin was induced by angiogenic factors such as TNF-α in vitro. Cy5.5 encapsulated into liposomes that were conjugated with an anti-E-selectin antibody successfully visualized Ehrlich ascites tumor cells when transplanted into mice. Thus, E-selectin targeting with liposomes containing a near-infrared fluorescent dye was found effective in visualizing tumors in vivo. This strategy should be extremely useful as a method to identify sentinel lymphatic nodes and angiogenic tumors as well as use for drug delivery to tumor cells.

元の言語English
ページ(範囲)70-77
ページ数8
ジャーナルContrast Media and Molecular Imaging
5
発行部数2
DOI
出版物ステータスPublished - 01-03-2010

    フィンガープリント

All Science Journal Classification (ASJC) codes

  • Radiology Nuclear Medicine and imaging

これを引用

Hirai, M., Hiramatsu, Y., Iwashita, S., Otani, T., Chen, L., Li, Y. G., Okada, M., Oie, K., Igarashi, K., Wakita, H., & Seno, M. (2010). E-selectin targeting to visualize tumors in vivo. Contrast Media and Molecular Imaging, 5(2), 70-77. https://doi.org/10.1002/cmmi.367