Effect of anticoagulants in colorimetric assay for basic carboxypeptidases

Hidefumi Komura, Kyoko Obata, William Campbell, Miho Yumoto, Yasuyo Shimomura, Hirotada Katsuya, Noriko Okada, Hidechika Okada

研究成果: Article査読

10 被引用数 (Scopus)

抄録

Carboxypeptidases (CP) in plasma and sera serve as regulators of anaphylatoxins such as C3a and C5a. The activity of CP can be measured by determining hippuric acid after cleavage of the small synthetic substrate hippuryl-L-arginine. Although a colorimetric assay is convenient for determining hippuric acid generated by CP, we noticed that some anticoagulants, such as citrate, interfere with the color development of the reagents used. EDTA and heparin provide an appropriate value. EGTA used as anticoagulant also provides an appropriate value. Therefore, concentration of citrate in samples should be controlled to be constant for background subtraction.

本文言語English
ページ(範囲)115-117
ページ数3
ジャーナルMICROBIOLOGY and IMMUNOLOGY
46
2
DOI
出版ステータスPublished - 2002
外部発表はい

All Science Journal Classification (ASJC) codes

  • 微生物学
  • 免疫学
  • ウイルス学

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