Effect of basic fibroblast growth factor on angiogenesis in the infarcted porcine heart

E. Watanabe, D. M. Smith, J. Sun, F. W. Smart, J. B. Delcarpio, T. B. Roberts, C. H. Van Meter, W. C. Claycomb

研究成果: ジャーナルへの寄稿学術論文査読

45 被引用数 (Scopus)

抄録

Administration of growth factors is emerging as a new therapeutic approach for the enhancement of collateral vessel formation in the ischemic heart. We have investigated the effects of intramyocardial delivery of FGF-2 in the presence and absence of heparin on angiogenesis in a porcine model of myocardial infarction. Yorkshire pigs were subjected to myocardial infarction by the placement of an embolization coil in the left anterior descending artery (n = 5). Four to five weeks after creation of an infarct, FGF-2 (10 μg) alone or in complex with heparin, heparan sulfate, or heparin agarose beads was injected either into the normal myocardium or along the infarct border area. Histologic evaluation of each injection site was performed 4 to 5 weeks post-injection. The effect of FGF-2 on angiogenesis was evaluated by determining the number of capillaries (diameter < 20 μm( and arterioles (> 20 μm with tunica media) in each area observed. The number of capillaries were not affected by the treatment of FGF-2 both in normal myocardium and infarct border area. However, in the normal myocardium, the number of arterioles were increased with the treatment of FGF-2 alone (85 ± 59%, P < 0.04), FGF-2 plus heparin (281 ± 193%, P < 0.004) and FGF-2-coated heparin beads (241 ± 141%, P < 0.01), as compared to control. Delivery of FGF-2 into the infarct border area, also increased the number of arterioles when FGF-2 was given with heparin (736 ± 154%, P < 0.001) or heparin heads (700 ± 109%, P < 0.001), as compared to control. FGF-2 administered with heparin was the most effective method of enhancing angiogenesis as compared to FGF-2 alone, FGF-2 plus heparan sulfate, or FGF-2 coated heparin agarose beads.

本文言語英語
ページ(範囲)30-37
ページ数8
ジャーナルBasic Research in Cardiology
93
1
DOI
出版ステータス出版済み - 02-1998
外部発表はい

All Science Journal Classification (ASJC) codes

  • 生理学
  • 循環器および心血管医学
  • 生理学(医学)

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