TY - JOUR
T1 - Effect of water-immersion restraint stress on tryptophan catabolism through the kynurenine pathway in rat tissues
AU - Ohta, Yoshiji
AU - Kubo, Hisako
AU - Yashiro, Koji
AU - Ohashi, Koji
AU - Tsuzuki, Yuji
AU - Wada, Naoya
AU - Yamamoto, Yasuko
AU - Saito, Kuniaki
N1 - Publisher Copyright:
© 2016, The Physiological Society of Japan and Springer Japan.
PY - 2017/5/1
Y1 - 2017/5/1
N2 - The aim of this study was to clarify the effect of water-immersion restraint stress (WIRS) on tryptophan (Trp) catabolism through the kynurenine (Kyn) pathway in rat tissues. The tissues of rats subjected to 6 h of WIRS (+WIRS) had increased tryptophan 2,3-dioxygenase (TDO) and indoleamine 2,3-dioxygenase (IDO) activities and increased TDO and IDO1 (one of two IDO isozymes in mammals) mRNA expression levels, with decreased Trp and increased Kyn contents in the liver. +WIRS rats had unchanged TDO and IDO activities in the kidney, decreased TDO activity and unchanged IDO activity in the brain, and unchanged IDO activity in the lung and spleen, with increased Kyn content in all of these tissues. Pretreatment of stressed rats with RU486, a glucocorticoid antagonist, attenuated the increased TOD activity, but not the increased IDO activity, with partial recoveries of the decreased Trp and increased Kyn contents in the liver. These results indicate that WIRS enhances hepatic Trp catabolism by inducing both IDO1 and TDO in rats.
AB - The aim of this study was to clarify the effect of water-immersion restraint stress (WIRS) on tryptophan (Trp) catabolism through the kynurenine (Kyn) pathway in rat tissues. The tissues of rats subjected to 6 h of WIRS (+WIRS) had increased tryptophan 2,3-dioxygenase (TDO) and indoleamine 2,3-dioxygenase (IDO) activities and increased TDO and IDO1 (one of two IDO isozymes in mammals) mRNA expression levels, with decreased Trp and increased Kyn contents in the liver. +WIRS rats had unchanged TDO and IDO activities in the kidney, decreased TDO activity and unchanged IDO activity in the brain, and unchanged IDO activity in the lung and spleen, with increased Kyn content in all of these tissues. Pretreatment of stressed rats with RU486, a glucocorticoid antagonist, attenuated the increased TOD activity, but not the increased IDO activity, with partial recoveries of the decreased Trp and increased Kyn contents in the liver. These results indicate that WIRS enhances hepatic Trp catabolism by inducing both IDO1 and TDO in rats.
KW - Glucocorticoid
KW - Indoleamine 2,3-Dioxygenase 1
KW - Interferon-γ
KW - Liver tryptophan catabolism
KW - Tryptophan 2,3-Dioxygenase
KW - Water-immersion restraint stress (rats)
UR - https://www.scopus.com/pages/publications/84976489030
UR - https://www.scopus.com/pages/publications/84976489030#tab=citedBy
U2 - 10.1007/s12576-016-0467-y
DO - 10.1007/s12576-016-0467-y
M3 - Article
C2 - 27364617
AN - SCOPUS:84976489030
SN - 1880-6546
VL - 67
SP - 361
EP - 372
JO - Journal of Physiological Sciences
JF - Journal of Physiological Sciences
IS - 3
ER -