Effect of xenotransplantation site on microrna expression of human colon cancer stem cells

Junko Mukohyama, Yohei Shimono, Kimihiro Yamashita, Yasuo Sumi, Toru Mukohara, Hironobu Minami, Yoshihiro Kakeji

研究成果: Article査読

7 被引用数 (Scopus)


Background: Cancer stem cells (CSCs) have a high tumorigenic ability to form patient-derived tumor xenografts (PDXs). PDXs are an attractive pre-clinical model, but gene expression and biological behavior of cancer cells in the tumor will change during establishment and passage of PDXs. Materials and Methods: Human colon cancer PDX was established and passaged either subcutaneously or orthotopically into the murine intestine. Histology and flow cytometric profile of the surgical specimen and the PDX were analyzed. CSCs were then isolated from the tumors and their microRNA (miRNA) expression was analyzed by semi-quantitative polymerase chain reaction. Results: The surgical specimens and PDXs were histologically similar. The size of CSC population increased and expression of miRNAs in CSCs changed in the passaged PDXs. Expression of oncogenic miRNAs was highly up-regulated in the CSCs of the orthotopically passaged PDXs. Conclusion: The xenotransplantation site and the number of tumor passages affect the miRNA expression of human colon CSCs.

ジャーナルAnticancer research
出版ステータスPublished - 2016

All Science Journal Classification (ASJC) codes

  • 腫瘍学
  • 癌研究


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