Effects of dopamine on N-terminus-deleted human tyrosine hydroxylase type 1 expressed in Escherichia coli

Akira Ota, Akira Nakashima, Keiji Mori, Toshiharu Nagatsu

研究成果: ジャーナルへの寄稿学術論文査読

15 被引用数 (Scopus)

抄録

N-Terminus deleted mutants and wild-type human tyrosine hydroxylase type I were expressed in Escherichia coli (E. coli) and utilized to investigate the dopamine-induced decrease in the enzyme catalytic activity and also to identify the specific portion in the N-terminus that affects the efficiency of the inhibitory action of dopamine. Supernatants of bacterial lysates were used as enzyme samples. The pH profiles of the enzyme catalytic activity were affected according to the degree of the deletion. The deletion up to 39 amino acid residues was enough to abolish the inhibitory effect of dopamine in the basic pH range. These results suggest that the inhibition by dopamine of tyrosine hydroxylase activity is closely related to the amino acid sequence in the N-terminus of the enzyme.

本文言語英語
ページ(範囲)57-60
ページ数4
ジャーナルNeuroscience Letters
229
1
DOI
出版ステータス出版済み - 20-06-1997

All Science Journal Classification (ASJC) codes

  • 神経科学一般

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