Effects of Picrotoxin Treatment on GABAA Receptor Supramolecular Complexes in Rat Brain

Yoshihisa Ito, Dong Koo Lim, Toshitaka Nabeshima, Ing K. Ho

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Abstract The effects of acute and chronic administration of a subconvulsive dose of picrotoxin on t‐[35S]butylbieyclophosphorothionate ([35S]TBPS), [3H]muscimol, and [3H]flunitrazepam binding characteristics in various regions and on the convulsant potency of picrotoxin in Sprague‐Dawley rats were examined. Acute administration of a sub‐convulsive dose of picrotoxin (3 mg/kg, i. p.) significantly increased [35S]TBPS and [3H]muscimol binding in cerebellum (CB) with no change in frontal cortex (FC). In rats treated chronically with picrotoxin (3 mg/kg, i.p., daily for 10 days), the Bmax of [35S]TBPS binding site was significantly decreased in the FC., striatum (ST), and CB with no change in KD values. Neither [3H]muscimol binding in the FC and CB nor [3H]fiunitrazepam binding in the FC was affected in these rats. In addition, the potency of pentobarbital to inhibit [35S]TBPS binding in vitro was not altered following acute or chronic treatment of picrotoxin. Chronic administration of picrotoxin did not affect convulsive ED50 or LD50 of picrotoxin; however, it delayed the onset of convulsions and increased the time to death. These results suggest that treatment with picrotoxin at a subconvulsive dose for 10 days causes down‐regulation of [35S]TBPS binding sites and that this down‐regulation might be related, at least in part, to the decreased extent of convulsant potency of picrotoxin. In addition, the results indicate possible interaction between convulsant binding sites and GABAA receptor sites in the CB following picrotoxin treatment.

元の言語English
ページ(範囲)1064-1070
ページ数7
ジャーナルJournal of neurochemistry
52
発行部数4
DOI
出版物ステータスPublished - 04-1989
外部発表Yes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Cellular and Molecular Neuroscience

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