Elimination of LMP1-expressing cells from a monolayer of gastric cancer AGS cells

Yoshitaka Sato, Shingo Ochiai, Takayuki Murata, Teru Kanda, Fumi Goshima, Hiroshi Kimura

研究成果: Article査読

6 被引用数 (Scopus)

抄録

Epstein-Barr virus (EBV) latently infects malignant epithelial cells in approximately 10% of all gastric cancers. Latent membrane protein 1 (LMP1), an oncogenic protein, plays an important role in malignant transformation in EBV-associated nasopharyngeal carcinoma and B-cell lymphoma; however, its expression has not been detected in EBVassociated gastric cancer. To address why LMP1 has not been detected in EBV-positive gastric tumors, we focused on the interactions between LMP1-positive and -negative cells and stably expressed LMP1 in the gastric cancer cell line AGS. We showed that the number of LMP1-positive cells decreased gradually with each cell passage when the cells were co-cultured with LMP1-negative cells. Time-lapse imaging showed that LMP1-positive cells were eliminated from a monolayer of LMP1-negative cells. Furthermore, LMP1-positive cells stimulated the proliferation of surrounding LMP1- negative cells, but not LMP1-positive cells, via exosome-mediated EGFR activation. Our data indicate that LMP1 expression drives cell competition between LMP1-positive and -negative cells, affecting the behavior of the cells within gastric tissue.

本文言語English
ページ(範囲)39345-39355
ページ数11
ジャーナルOncotarget
8
24
DOI
出版ステータスPublished - 2017
外部発表はい

All Science Journal Classification (ASJC) codes

  • Oncology

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