Endothelin-1 stimulates sodium-dependent calcium efflux from bovine adrenal chromaffin cells in culture

Hitoshi Houchi, Masanori Yoshizumi, Koichiro Tsuchiya, Masao Hirose, Tetsuya Kitagawa, Toshihide Kujime, Eiji Shimizu, Yasuharu Niwa, Kazuo Minakuchi, Toshiaki Tamaki

研究成果: Article査読

9 被引用数 (Scopus)

抄録

1. The effect of endothelin (ET)-1 on Ca2+ efflux from cultured bovine adrenal chromaffin cells was examined. ET-1 (10-7 M) significantly increased intracellular free Ca2+ level ([Ca2+](i)), 45Ca2+ uptake and catecholamine secretion in the cells. 2. ET-1 stimulated the efflux of 45Ca2+ from the cells preloaded with 45Ca2+ in a concentration-dependent manner (10-9-10-7 M). This stimulatory effect was inhibited by ET(B) receptor antagonist BQ788, but not by ET(A) receptor antagonist BQ123. Selective ET(B) receptor agonists Suc-[Glu9 Ala11,15]-ET-1 and sarafotoxin S6c (SRTX) also stimulated 45Ca2+ efflux from the cells. 3. ET-1, Suc-[Glu9, Ala11,15]-ET-1 and SRTX increased the level of cyclic GMP in the adrenal chromaffin cells. ET-1 induced an increase in the nitric oxide (NO) level in the cells. The stimulatory effects by which ET-1 increases NO level and 45Ca2+ efflux were inhibited by N(G)-monomethyl-L-arginine acetate (L-NMMA), a competitive inhibitor of NO synthase. 4. The 45Ca2+ efflux stimulated by ET-1 was inhibited by deprivation of extracellular Na+, but not by deprivation of Ca2+. 5. These results suggest that ET-1 stimulates an extracellular Na+-dependent Ca2+ efflux through the activation of NO synthase in cultured bovine adrenal chromaffin cells.

本文言語English
ページ(範囲)55-60
ページ数6
ジャーナルBritish Journal of Pharmacology
125
1
DOI
出版ステータスPublished - 1998

All Science Journal Classification (ASJC) codes

  • 薬理学

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