Enhanced expression of MYCN leads to centrosome hyperamplification after DNA damage in neuroblastoma cells

Eiji Sugihara, Masayuki Kanai, Akira Matsui, Masafumi Onodera, Manfred Schwab, Masanao Miwa

研究成果: Article査読

14 被引用数 (Scopus)

抄録

Centrosomes play important roles in cell polarity, regulation of cell cycle and chromosomal stability. Centrosome abnormality is frequently found in many cancers and contributes to chromosomal instability (including aneuploidy, tetraploidy, and/or micronuclei) in daughter cells through the assembly of multipolar or monopolar spindles during mitosis. It has recently been reported that loss of tumor suppressor genes or overexpression of oncogenes causes centrosome hyperamplification. Amplification and overexpression of the MYCN oncogene is found in a subgroup of neuroblastomas. In this study, we examined whether overexpression of MYCN causes centrosome hyperamplification in neuroblastoma cells. We show that ectopic expression of MYCN alone in a neuroblastoma cell line did not cause centrosome hyperamplification. However, centrosome hyperamplification and micronuclei formation were seen in these cells after DNA damage. These findings suggest that overexpression of MYCN abrogates the regulation of the centrosome cycle after DNA damage.

本文言語English
ページ(範囲)1005-1009
ページ数5
ジャーナルOncogene
23
4
DOI
出版ステータスPublished - 29-01-2004
外部発表はい

All Science Journal Classification (ASJC) codes

  • 分子生物学
  • 遺伝学
  • 癌研究

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