TY - JOUR
T1 - Enhancement of fibroblast growth factor-induced diacylglycerol formation and protein kinase C activation by colon tumor-promoting bile acid in Swiss 3T3 cells. Different modes of action between bile acid and phorbol ester
AU - Takeyama, Yoshifumi
AU - Tanimoto, Tetsuji
AU - Hoshijima, Masahiko
AU - Kaibuchi, Kozo
AU - Ohyanagi, Harumasa
AU - Saitoh, Yoichi
AU - Takai, Yoshimi
N1 - Funding Information:
This investigationw as supported in part by researchg rantsf rom the ScientificR esearchF unds of the Ministry of Education,S ciencea nd Culture, Japan (1985),I nvestigationC ommitteeo n Abnormalities in Hormone Receptor Mechanism, the Ministry of Health and Welfare, Japan (198% the Yamanouchi Foundation for Research on Metabolic Disorders( 1985),R esearchF oundation for Cancera nd CardiovascularD iseases(1 985)a nd Mitsukoshi Prize for Medicine( 1985).T he authors are grateful to Miss Junko Yamaguchi for her skillful secretariaal ssistance.
PY - 1986/3/3
Y1 - 1986/3/3
N2 - A small amount (50-200 μM) of deoxycholate (DOC), a colon tumor-promoting bile acid, did not show a direct effect on protein kinase C activity in a cell-free system, but enhanced fibroblast growth factor (FGF)-induced diacylglycerol formation and protein kinase C activation in Swiss 3T3 cells. DOC potentiated both reactions induced by submaximal doses of FGF but showed little effect on the maximal levels of the reactions. DOC alone was inactive in eliciting both reactions in the absence of FGF. DOC did not affect the binding of FGF to the cells. Since it has been described that diacylglycerol serves as a messenger for the activation of protein kinase C in the action of FGF in Swiss 3T3 cells [(1985) FEBS Lett. 191, 205-210], these results suggest that a small amount of DOC increases the sensitivity to FGF of diacylglycerol formation and thereby potentiates protein kinase C activation in this cell line. This action of DOC was in marked contrast to that of 12-O-tetradecanoylphorbol-13-acetate, a potent tumor-promoting phorbol ester, which directly activated protein kinase C in cell-free and intact cell systems.
AB - A small amount (50-200 μM) of deoxycholate (DOC), a colon tumor-promoting bile acid, did not show a direct effect on protein kinase C activity in a cell-free system, but enhanced fibroblast growth factor (FGF)-induced diacylglycerol formation and protein kinase C activation in Swiss 3T3 cells. DOC potentiated both reactions induced by submaximal doses of FGF but showed little effect on the maximal levels of the reactions. DOC alone was inactive in eliciting both reactions in the absence of FGF. DOC did not affect the binding of FGF to the cells. Since it has been described that diacylglycerol serves as a messenger for the activation of protein kinase C in the action of FGF in Swiss 3T3 cells [(1985) FEBS Lett. 191, 205-210], these results suggest that a small amount of DOC increases the sensitivity to FGF of diacylglycerol formation and thereby potentiates protein kinase C activation in this cell line. This action of DOC was in marked contrast to that of 12-O-tetradecanoylphorbol-13-acetate, a potent tumor-promoting phorbol ester, which directly activated protein kinase C in cell-free and intact cell systems.
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U2 - 10.1016/0014-5793(86)80353-2
DO - 10.1016/0014-5793(86)80353-2
M3 - Article
C2 - 3949019
AN - SCOPUS:0022554018
SN - 0014-5793
VL - 197
SP - 339
EP - 343
JO - FEBS Letters
JF - FEBS Letters
IS - 1-2
ER -