Enhancement of individual differences in proliferation and differentiation potentials of aged human adipose-derived stem cells

Mika Kawagishi-Hotta, Seiji Hasegawa, Toshio Igarashi, Takaaki Yamada, Masayuki Takahashi, Shigeki Numata, Tsukane Kobayashi, Yohei Iwata, Masaru Arima, Naoki Yamamoto, Akiko Yagami, Satoru Nakata, Tohru Uzawa, Kayoko Matsunaga, Kazumitsu Sugiura, Hirohiko Akamatsu

研究成果: Article

3 引用 (Scopus)

抄録

Background Adipose-derived stem cells (ASCs) are a robust, multipotent cell source. They are easily obtained and hold promise in many regenerative applications. It is generally considered that the function of somatic stem cells declines with age. Although several studies have examined the effects of donor age on proliferation potential and pluripotency of ASCs, the results of these studies were not consistent. Objective This study tested whether the donor age affects the yield of ASCs from adipose tissue, as well as the proliferation and differentiation potentials of ASCs. Methods This study used ASCs obtained from adipose tissues of 260 donors (ages 5–97 years). ASCs were examined for individual differences in proliferation, and adipogenic, osteogenic and chondrogenic differentiation potentials in vitro. Characteristics of ASCs from each donor were evaluated by the principal component analysis (PCA) using their potential parameters. Results Analyses on ASCs demonstrated that adipogenic potentials declined with age, but proliferation, osteogenic and chondrogenic potentials were not correlated with age. Interestingly, in all ASC potentials, including adipogenesis, individual differences were observed. Principal component analysis (PCA) revealed that individual differences became evident in the elderly, and those variations were more prominent in females than in males. Conclusions This study demonstrated age-related changes in the potentials of ASCs and revealed that the individual differences of ASCs become significant in people over 60 years of age (for females over 60, and for males over 80). We believe that it is important to carefully observe ASC potentials in order to achieve effective regenerative medicine treatments using ASCs.

元の言語English
ページ(範囲)29-40
ページ数12
ジャーナルRegenerative Therapy
6
DOI
出版物ステータスPublished - 01-06-2017

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Stem cells
Individuality
Stem Cells
Tissue Donors
Principal Component Analysis
Adipose Tissue
Principal component analysis
Tissue
Adipogenesis
Adult Stem Cells
Regenerative Medicine

All Science Journal Classification (ASJC) codes

  • Biomaterials
  • Biomedical Engineering
  • Developmental Biology

これを引用

Kawagishi-Hotta, M., Hasegawa, S., Igarashi, T., Yamada, T., Takahashi, M., Numata, S., ... Akamatsu, H. (2017). Enhancement of individual differences in proliferation and differentiation potentials of aged human adipose-derived stem cells. Regenerative Therapy, 6, 29-40. https://doi.org/10.1016/j.reth.2016.12.004
Kawagishi-Hotta, Mika ; Hasegawa, Seiji ; Igarashi, Toshio ; Yamada, Takaaki ; Takahashi, Masayuki ; Numata, Shigeki ; Kobayashi, Tsukane ; Iwata, Yohei ; Arima, Masaru ; Yamamoto, Naoki ; Yagami, Akiko ; Nakata, Satoru ; Uzawa, Tohru ; Matsunaga, Kayoko ; Sugiura, Kazumitsu ; Akamatsu, Hirohiko. / Enhancement of individual differences in proliferation and differentiation potentials of aged human adipose-derived stem cells. :: Regenerative Therapy. 2017 ; 巻 6. pp. 29-40.
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title = "Enhancement of individual differences in proliferation and differentiation potentials of aged human adipose-derived stem cells",
abstract = "Background Adipose-derived stem cells (ASCs) are a robust, multipotent cell source. They are easily obtained and hold promise in many regenerative applications. It is generally considered that the function of somatic stem cells declines with age. Although several studies have examined the effects of donor age on proliferation potential and pluripotency of ASCs, the results of these studies were not consistent. Objective This study tested whether the donor age affects the yield of ASCs from adipose tissue, as well as the proliferation and differentiation potentials of ASCs. Methods This study used ASCs obtained from adipose tissues of 260 donors (ages 5–97 years). ASCs were examined for individual differences in proliferation, and adipogenic, osteogenic and chondrogenic differentiation potentials in vitro. Characteristics of ASCs from each donor were evaluated by the principal component analysis (PCA) using their potential parameters. Results Analyses on ASCs demonstrated that adipogenic potentials declined with age, but proliferation, osteogenic and chondrogenic potentials were not correlated with age. Interestingly, in all ASC potentials, including adipogenesis, individual differences were observed. Principal component analysis (PCA) revealed that individual differences became evident in the elderly, and those variations were more prominent in females than in males. Conclusions This study demonstrated age-related changes in the potentials of ASCs and revealed that the individual differences of ASCs become significant in people over 60 years of age (for females over 60, and for males over 80). We believe that it is important to carefully observe ASC potentials in order to achieve effective regenerative medicine treatments using ASCs.",
author = "Mika Kawagishi-Hotta and Seiji Hasegawa and Toshio Igarashi and Takaaki Yamada and Masayuki Takahashi and Shigeki Numata and Tsukane Kobayashi and Yohei Iwata and Masaru Arima and Naoki Yamamoto and Akiko Yagami and Satoru Nakata and Tohru Uzawa and Kayoko Matsunaga and Kazumitsu Sugiura and Hirohiko Akamatsu",
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Kawagishi-Hotta, M, Hasegawa, S, Igarashi, T, Yamada, T, Takahashi, M, Numata, S, Kobayashi, T, Iwata, Y, Arima, M, Yamamoto, N, Yagami, A, Nakata, S, Uzawa, T, Matsunaga, K, Sugiura, K & Akamatsu, H 2017, 'Enhancement of individual differences in proliferation and differentiation potentials of aged human adipose-derived stem cells', Regenerative Therapy, 巻. 6, pp. 29-40. https://doi.org/10.1016/j.reth.2016.12.004

Enhancement of individual differences in proliferation and differentiation potentials of aged human adipose-derived stem cells. / Kawagishi-Hotta, Mika; Hasegawa, Seiji; Igarashi, Toshio; Yamada, Takaaki; Takahashi, Masayuki; Numata, Shigeki; Kobayashi, Tsukane; Iwata, Yohei; Arima, Masaru; Yamamoto, Naoki; Yagami, Akiko; Nakata, Satoru; Uzawa, Tohru; Matsunaga, Kayoko; Sugiura, Kazumitsu; Akamatsu, Hirohiko.

:: Regenerative Therapy, 巻 6, 01.06.2017, p. 29-40.

研究成果: Article

TY - JOUR

T1 - Enhancement of individual differences in proliferation and differentiation potentials of aged human adipose-derived stem cells

AU - Kawagishi-Hotta, Mika

AU - Hasegawa, Seiji

AU - Igarashi, Toshio

AU - Yamada, Takaaki

AU - Takahashi, Masayuki

AU - Numata, Shigeki

AU - Kobayashi, Tsukane

AU - Iwata, Yohei

AU - Arima, Masaru

AU - Yamamoto, Naoki

AU - Yagami, Akiko

AU - Nakata, Satoru

AU - Uzawa, Tohru

AU - Matsunaga, Kayoko

AU - Sugiura, Kazumitsu

AU - Akamatsu, Hirohiko

PY - 2017/6/1

Y1 - 2017/6/1

N2 - Background Adipose-derived stem cells (ASCs) are a robust, multipotent cell source. They are easily obtained and hold promise in many regenerative applications. It is generally considered that the function of somatic stem cells declines with age. Although several studies have examined the effects of donor age on proliferation potential and pluripotency of ASCs, the results of these studies were not consistent. Objective This study tested whether the donor age affects the yield of ASCs from adipose tissue, as well as the proliferation and differentiation potentials of ASCs. Methods This study used ASCs obtained from adipose tissues of 260 donors (ages 5–97 years). ASCs were examined for individual differences in proliferation, and adipogenic, osteogenic and chondrogenic differentiation potentials in vitro. Characteristics of ASCs from each donor were evaluated by the principal component analysis (PCA) using their potential parameters. Results Analyses on ASCs demonstrated that adipogenic potentials declined with age, but proliferation, osteogenic and chondrogenic potentials were not correlated with age. Interestingly, in all ASC potentials, including adipogenesis, individual differences were observed. Principal component analysis (PCA) revealed that individual differences became evident in the elderly, and those variations were more prominent in females than in males. Conclusions This study demonstrated age-related changes in the potentials of ASCs and revealed that the individual differences of ASCs become significant in people over 60 years of age (for females over 60, and for males over 80). We believe that it is important to carefully observe ASC potentials in order to achieve effective regenerative medicine treatments using ASCs.

AB - Background Adipose-derived stem cells (ASCs) are a robust, multipotent cell source. They are easily obtained and hold promise in many regenerative applications. It is generally considered that the function of somatic stem cells declines with age. Although several studies have examined the effects of donor age on proliferation potential and pluripotency of ASCs, the results of these studies were not consistent. Objective This study tested whether the donor age affects the yield of ASCs from adipose tissue, as well as the proliferation and differentiation potentials of ASCs. Methods This study used ASCs obtained from adipose tissues of 260 donors (ages 5–97 years). ASCs were examined for individual differences in proliferation, and adipogenic, osteogenic and chondrogenic differentiation potentials in vitro. Characteristics of ASCs from each donor were evaluated by the principal component analysis (PCA) using their potential parameters. Results Analyses on ASCs demonstrated that adipogenic potentials declined with age, but proliferation, osteogenic and chondrogenic potentials were not correlated with age. Interestingly, in all ASC potentials, including adipogenesis, individual differences were observed. Principal component analysis (PCA) revealed that individual differences became evident in the elderly, and those variations were more prominent in females than in males. Conclusions This study demonstrated age-related changes in the potentials of ASCs and revealed that the individual differences of ASCs become significant in people over 60 years of age (for females over 60, and for males over 80). We believe that it is important to carefully observe ASC potentials in order to achieve effective regenerative medicine treatments using ASCs.

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