Epigenetic barrier against the propagation of fluctuating gene expression in embryonic stem cells

Yuki Saito; Akira Kunitomi; Tomohisa Seki; Shugo Tohyama; Dai Kusumoto; Makoto Takei; Shin Kashimura; Hisayuki Hashimoto; Gakuto Yozu; Chikaaki Motoda; Masaya Shimojima; Toru Egashira; Mayumi Oda; Keiichi Fukuda; Shinsuke Yuasa

doi:10.1002/1873-3468.12791

Epigenetic barrier against the propagation of fluctuating gene expression in embryonic stem cells

Yuki Saito
, Akira Kunitomi
, Tomohisa Seki
, Shugo Tohyama
, Dai Kusumoto
, Makoto Takei
, Shin Kashimura
, Hisayuki Hashimoto
, Gakuto Yozu
, Chikaaki Motoda
, Masaya Shimojima
, Toru Egashira
, Mayumi Oda
, Keiichi Fukuda
, Shinsuke Yuasa

研究成果: ジャーナルへの寄稿 › 学術論文 › 査読

抄録

The expression of pluripotency genes fluctuates in a population of embryonic stem (ES) cells and the fluctuations in the expression of some pluripotency genes correlate. However, no correlation in the fluctuation of Pou5f1, Zfp42, and Nanog expression was observed in ES cells. Correlation between Pou5f1 and Zfp42 fluctuations was demonstrated in ES cells containing a knockout in the NuRD component Mbd3. ES cells containing a triple knockout in the DNA methyltransferases Dnmt1, Dnmt3a, and Dnmt3b showed correlation between the fluctuation of Pou5f1, Zfp42, and Nanog gene expression. We suggest that an epigenetic barrier is key to preventing the propagation of fluctuating pluripotency gene expression in ES cells.

本文言語	英語
ページ（範囲）	2879-2889
ページ数	11
ジャーナル	FEBS Letters
巻	591
号	18
DOI	https://doi.org/10.1002/1873-3468.12791
出版ステータス	出版済み - 09-2017
外部発表	はい

All Science Journal Classification (ASJC) codes

生物理学
構造生物学
生化学
分子生物学
遺伝学
細胞生物学

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10.1002/1873-3468.12791

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title = "Epigenetic barrier against the propagation of fluctuating gene expression in embryonic stem cells",

abstract = "The expression of pluripotency genes fluctuates in a population of embryonic stem (ES) cells and the fluctuations in the expression of some pluripotency genes correlate. However, no correlation in the fluctuation of Pou5f1, Zfp42, and Nanog expression was observed in ES cells. Correlation between Pou5f1 and Zfp42 fluctuations was demonstrated in ES cells containing a knockout in the NuRD component Mbd3. ES cells containing a triple knockout in the DNA methyltransferases Dnmt1, Dnmt3a, and Dnmt3b showed correlation between the fluctuation of Pou5f1, Zfp42, and Nanog gene expression. We suggest that an epigenetic barrier is key to preventing the propagation of fluctuating pluripotency gene expression in ES cells.",

author = "Yuki Saito and Akira Kunitomi and Tomohisa Seki and Shugo Tohyama and Dai Kusumoto and Makoto Takei and Shin Kashimura and Hisayuki Hashimoto and Gakuto Yozu and Chikaaki Motoda and Masaya Shimojima and Toru Egashira and Mayumi Oda and Keiichi Fukuda and Shinsuke Yuasa",

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T1 - Epigenetic barrier against the propagation of fluctuating gene expression in embryonic stem cells

AU - Saito, Yuki

AU - Kunitomi, Akira

AU - Seki, Tomohisa

AU - Tohyama, Shugo

AU - Kusumoto, Dai

AU - Takei, Makoto

AU - Kashimura, Shin

AU - Hashimoto, Hisayuki

AU - Yozu, Gakuto

AU - Motoda, Chikaaki

AU - Shimojima, Masaya

AU - Egashira, Toru

AU - Oda, Mayumi

AU - Fukuda, Keiichi

AU - Yuasa, Shinsuke

PY - 2017/9

Y1 - 2017/9

N2 - The expression of pluripotency genes fluctuates in a population of embryonic stem (ES) cells and the fluctuations in the expression of some pluripotency genes correlate. However, no correlation in the fluctuation of Pou5f1, Zfp42, and Nanog expression was observed in ES cells. Correlation between Pou5f1 and Zfp42 fluctuations was demonstrated in ES cells containing a knockout in the NuRD component Mbd3. ES cells containing a triple knockout in the DNA methyltransferases Dnmt1, Dnmt3a, and Dnmt3b showed correlation between the fluctuation of Pou5f1, Zfp42, and Nanog gene expression. We suggest that an epigenetic barrier is key to preventing the propagation of fluctuating pluripotency gene expression in ES cells.

AB - The expression of pluripotency genes fluctuates in a population of embryonic stem (ES) cells and the fluctuations in the expression of some pluripotency genes correlate. However, no correlation in the fluctuation of Pou5f1, Zfp42, and Nanog expression was observed in ES cells. Correlation between Pou5f1 and Zfp42 fluctuations was demonstrated in ES cells containing a knockout in the NuRD component Mbd3. ES cells containing a triple knockout in the DNA methyltransferases Dnmt1, Dnmt3a, and Dnmt3b showed correlation between the fluctuation of Pou5f1, Zfp42, and Nanog gene expression. We suggest that an epigenetic barrier is key to preventing the propagation of fluctuating pluripotency gene expression in ES cells.

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SN - 0014-5793

VL - 591

SP - 2879

EP - 2889

JO - FEBS Letters

JF - FEBS Letters

IS - 18

ER -

Epigenetic barrier against the propagation of fluctuating gene expression in embryonic stem cells

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