Epithelial-to-mesenchymal transition in cyst lining epithelial cells in an orthologous PCK rat model of autosomal-recessive polycystic kidney disease

Hiroko Togawa, Koichi Nakanishi, Hironobu Mukaiyama, Taketsugu Hama, Yuko Shima, Mayumi Sako, Masayasu Miyajima, Kandai Nozu, Kazuhiro Nishii, Shizuko Nagao, Hisahide Takahashi, Kazumoto Iijima, Norishige Yoshikawa

研究成果: Article

30 引用 (Scopus)

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In polycystic kidney disease (PKD), cyst lining cells show polarity abnormalities. Recent studies have demonstrated loss of cell contact in cyst cells, suggesting induction of epithelial-to-mesenchymal transition (EMT). Recently, EMT has been implicated in the pathogenesis of PKD. To explore further evidence of EMT in PKD, we examined age- and segment-specific expression of adhesion molecules and mesenchymal markers in PCK rats, an orthologous model of human autosomal-recessive PKD. Kidneys from 5 male PCK and 5 control rats each at 0 days, 1, 3, 10, and 14 wk, and 4 mo of age were serially sectioned and stained with segment-specific markers and antibodies against E-cadherin, Snail1, β-catenin, and N-cadherin. mRNAs for E-cadherin and Snail1 were quantified by real-time PCR. Vimentin, fibronectin, and α-smooth muscle actin (α-SMA) expressions were assessed as mesenchymal markers. E-cadherin expression pattern was correlated with the disease pathology in that tubule segments showing the highest expression in control had much severer cyst formation in PCK rats. In PCK rats, E-cadherin and β-catenin in cystic tubules was attenuated and localized to lateral areas of cell-cell contact, whereas nuclear expression of Snail1 increased in parallel with cyst enlargement. Some epithelial cells in large cysts derived from these segments, especially in adjacent fibrotic areas, showed positive immunoreactivity for vimentin and fibronectin. In conclusion, these findings suggest that epithelial cells in cysts acquire mesenchymal features in response to cyst enlargement and participate in progressive renal fibrosis. Our study clarified the nephron segment-specific cyst profile related to EMT in PCK rats. EMT may play a key role in polycystic kidney disease.

元の言語English
ジャーナルAmerican Journal of Physiology - Renal Physiology
300
発行部数2
DOI
出版物ステータスPublished - 01-02-2011

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Autosomal Recessive Polycystic Kidney
Epithelial-Mesenchymal Transition
Cysts
Epithelial Cells
Polycystic Kidney Diseases
Cadherins
Catenins
Vimentin
Fibronectins
Kidney
Cell Polarity
Nephrons
Smooth Muscle
Actins
Real-Time Polymerase Chain Reaction
Fibrosis
Pathology

All Science Journal Classification (ASJC) codes

  • Physiology
  • Urology

これを引用

Togawa, Hiroko ; Nakanishi, Koichi ; Mukaiyama, Hironobu ; Hama, Taketsugu ; Shima, Yuko ; Sako, Mayumi ; Miyajima, Masayasu ; Nozu, Kandai ; Nishii, Kazuhiro ; Nagao, Shizuko ; Takahashi, Hisahide ; Iijima, Kazumoto ; Yoshikawa, Norishige. / Epithelial-to-mesenchymal transition in cyst lining epithelial cells in an orthologous PCK rat model of autosomal-recessive polycystic kidney disease. :: American Journal of Physiology - Renal Physiology. 2011 ; 巻 300, 番号 2.
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abstract = "In polycystic kidney disease (PKD), cyst lining cells show polarity abnormalities. Recent studies have demonstrated loss of cell contact in cyst cells, suggesting induction of epithelial-to-mesenchymal transition (EMT). Recently, EMT has been implicated in the pathogenesis of PKD. To explore further evidence of EMT in PKD, we examined age- and segment-specific expression of adhesion molecules and mesenchymal markers in PCK rats, an orthologous model of human autosomal-recessive PKD. Kidneys from 5 male PCK and 5 control rats each at 0 days, 1, 3, 10, and 14 wk, and 4 mo of age were serially sectioned and stained with segment-specific markers and antibodies against E-cadherin, Snail1, β-catenin, and N-cadherin. mRNAs for E-cadherin and Snail1 were quantified by real-time PCR. Vimentin, fibronectin, and α-smooth muscle actin (α-SMA) expressions were assessed as mesenchymal markers. E-cadherin expression pattern was correlated with the disease pathology in that tubule segments showing the highest expression in control had much severer cyst formation in PCK rats. In PCK rats, E-cadherin and β-catenin in cystic tubules was attenuated and localized to lateral areas of cell-cell contact, whereas nuclear expression of Snail1 increased in parallel with cyst enlargement. Some epithelial cells in large cysts derived from these segments, especially in adjacent fibrotic areas, showed positive immunoreactivity for vimentin and fibronectin. In conclusion, these findings suggest that epithelial cells in cysts acquire mesenchymal features in response to cyst enlargement and participate in progressive renal fibrosis. Our study clarified the nephron segment-specific cyst profile related to EMT in PCK rats. EMT may play a key role in polycystic kidney disease.",
author = "Hiroko Togawa and Koichi Nakanishi and Hironobu Mukaiyama and Taketsugu Hama and Yuko Shima and Mayumi Sako and Masayasu Miyajima and Kandai Nozu and Kazuhiro Nishii and Shizuko Nagao and Hisahide Takahashi and Kazumoto Iijima and Norishige Yoshikawa",
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Togawa, H, Nakanishi, K, Mukaiyama, H, Hama, T, Shima, Y, Sako, M, Miyajima, M, Nozu, K, Nishii, K, Nagao, S, Takahashi, H, Iijima, K & Yoshikawa, N 2011, 'Epithelial-to-mesenchymal transition in cyst lining epithelial cells in an orthologous PCK rat model of autosomal-recessive polycystic kidney disease', American Journal of Physiology - Renal Physiology, 巻. 300, 番号 2. https://doi.org/10.1152/ajprenal.00038.2010

Epithelial-to-mesenchymal transition in cyst lining epithelial cells in an orthologous PCK rat model of autosomal-recessive polycystic kidney disease. / Togawa, Hiroko; Nakanishi, Koichi; Mukaiyama, Hironobu; Hama, Taketsugu; Shima, Yuko; Sako, Mayumi; Miyajima, Masayasu; Nozu, Kandai; Nishii, Kazuhiro; Nagao, Shizuko; Takahashi, Hisahide; Iijima, Kazumoto; Yoshikawa, Norishige.

:: American Journal of Physiology - Renal Physiology, 巻 300, 番号 2, 01.02.2011.

研究成果: Article

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T1 - Epithelial-to-mesenchymal transition in cyst lining epithelial cells in an orthologous PCK rat model of autosomal-recessive polycystic kidney disease

AU - Togawa, Hiroko

AU - Nakanishi, Koichi

AU - Mukaiyama, Hironobu

AU - Hama, Taketsugu

AU - Shima, Yuko

AU - Sako, Mayumi

AU - Miyajima, Masayasu

AU - Nozu, Kandai

AU - Nishii, Kazuhiro

AU - Nagao, Shizuko

AU - Takahashi, Hisahide

AU - Iijima, Kazumoto

AU - Yoshikawa, Norishige

PY - 2011/2/1

Y1 - 2011/2/1

N2 - In polycystic kidney disease (PKD), cyst lining cells show polarity abnormalities. Recent studies have demonstrated loss of cell contact in cyst cells, suggesting induction of epithelial-to-mesenchymal transition (EMT). Recently, EMT has been implicated in the pathogenesis of PKD. To explore further evidence of EMT in PKD, we examined age- and segment-specific expression of adhesion molecules and mesenchymal markers in PCK rats, an orthologous model of human autosomal-recessive PKD. Kidneys from 5 male PCK and 5 control rats each at 0 days, 1, 3, 10, and 14 wk, and 4 mo of age were serially sectioned and stained with segment-specific markers and antibodies against E-cadherin, Snail1, β-catenin, and N-cadherin. mRNAs for E-cadherin and Snail1 were quantified by real-time PCR. Vimentin, fibronectin, and α-smooth muscle actin (α-SMA) expressions were assessed as mesenchymal markers. E-cadherin expression pattern was correlated with the disease pathology in that tubule segments showing the highest expression in control had much severer cyst formation in PCK rats. In PCK rats, E-cadherin and β-catenin in cystic tubules was attenuated and localized to lateral areas of cell-cell contact, whereas nuclear expression of Snail1 increased in parallel with cyst enlargement. Some epithelial cells in large cysts derived from these segments, especially in adjacent fibrotic areas, showed positive immunoreactivity for vimentin and fibronectin. In conclusion, these findings suggest that epithelial cells in cysts acquire mesenchymal features in response to cyst enlargement and participate in progressive renal fibrosis. Our study clarified the nephron segment-specific cyst profile related to EMT in PCK rats. EMT may play a key role in polycystic kidney disease.

AB - In polycystic kidney disease (PKD), cyst lining cells show polarity abnormalities. Recent studies have demonstrated loss of cell contact in cyst cells, suggesting induction of epithelial-to-mesenchymal transition (EMT). Recently, EMT has been implicated in the pathogenesis of PKD. To explore further evidence of EMT in PKD, we examined age- and segment-specific expression of adhesion molecules and mesenchymal markers in PCK rats, an orthologous model of human autosomal-recessive PKD. Kidneys from 5 male PCK and 5 control rats each at 0 days, 1, 3, 10, and 14 wk, and 4 mo of age were serially sectioned and stained with segment-specific markers and antibodies against E-cadherin, Snail1, β-catenin, and N-cadherin. mRNAs for E-cadherin and Snail1 were quantified by real-time PCR. Vimentin, fibronectin, and α-smooth muscle actin (α-SMA) expressions were assessed as mesenchymal markers. E-cadherin expression pattern was correlated with the disease pathology in that tubule segments showing the highest expression in control had much severer cyst formation in PCK rats. In PCK rats, E-cadherin and β-catenin in cystic tubules was attenuated and localized to lateral areas of cell-cell contact, whereas nuclear expression of Snail1 increased in parallel with cyst enlargement. Some epithelial cells in large cysts derived from these segments, especially in adjacent fibrotic areas, showed positive immunoreactivity for vimentin and fibronectin. In conclusion, these findings suggest that epithelial cells in cysts acquire mesenchymal features in response to cyst enlargement and participate in progressive renal fibrosis. Our study clarified the nephron segment-specific cyst profile related to EMT in PCK rats. EMT may play a key role in polycystic kidney disease.

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DO - 10.1152/ajprenal.00038.2010

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