Epstein-barr virus BBRF2 is required for maximum infectivity

H. M.Abdullah Al Masud, Yusuke Yanagi, Takahiro Watanabe, Yoshitaka Sato, Hiroshi Kimura, Takayuki Murata

研究成果: Article査読

3 被引用数 (Scopus)

抄録

Epstein-Barr virus (EBV) is a member of the gammaherpesvirinae, which causes infectious mononucleosis and several types of cancer. BBRF2 is an uncharacterized gene of EBV and is expressed during the lytic phase. To evaluate its function, BBRF2-knockout EBV was prepared using bacterial artificial chromosome (BAC) technology and the CRISPR/Cas9 system. Although viral gene expression, DNA synthesis, and progeny secretion were not affected, the infectivity of progeny viruses was significantly reduced by the disruption of BBRF2. When expressed alone, BBRF2 protein localized to the nucleus and cytoplasm, while the coexpression of an interacting partner, BSRF1, resulted in its relocalization to the cytoplasm. Interestingly, the coexpression of BBRF2 protected BSRF1 from proteasome/ubiquitin-dependent degradation. Therefore, BBRF2, together with BSRF1, augments viral infectivity.

本文言語English
論文番号705
ジャーナルMicroorganisms
7
12
DOI
出版ステータスPublished - 12-2019

All Science Journal Classification (ASJC) codes

  • 微生物学
  • ウイルス学
  • 微生物学(医療)

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