TY - JOUR
T1 - Escherichia coli Producing CTX-M-2 β-Lactamase in Cattle, Japan
AU - Shiraki, Yutaka
AU - Shibata, Naohiro
AU - Doi, Yohei
AU - Arakawa, Yoshichika
PY - 2004/1
Y1 - 2004/1
N2 - From November 2000 to June 2001, Escherichia coli strains producing CTX-M-2 β-lactamase were isolated from 6 (1.5%) of 396 cattle fecal samples and 2 (0.7%) of 270 surface swabs of cattle carcasses in Japan. The blaCTX-M-2 gene responsible for CTX-M-2 production was encoded on transferable plasmids, and the gene was transferred to E. coli CSH2 with a very high frequency (2 × 10-4 to 6 × 10-1 per donor cells) by conjugation. Random amplified polymorphic DNA analysis of nine isolates showed at least five different patterns. These findings suggest that CTX-M-2 producers might have originated from cattle through the use of cephalosporins such as ceftiofur and that cattle could be a reservoir of CTX-M-2-producing E. coli. Continuous and strategic surveillance of antimicrobial-resistant bacteria in livestock is essential to suppress further dissemination of these bacteria into society at large.
AB - From November 2000 to June 2001, Escherichia coli strains producing CTX-M-2 β-lactamase were isolated from 6 (1.5%) of 396 cattle fecal samples and 2 (0.7%) of 270 surface swabs of cattle carcasses in Japan. The blaCTX-M-2 gene responsible for CTX-M-2 production was encoded on transferable plasmids, and the gene was transferred to E. coli CSH2 with a very high frequency (2 × 10-4 to 6 × 10-1 per donor cells) by conjugation. Random amplified polymorphic DNA analysis of nine isolates showed at least five different patterns. These findings suggest that CTX-M-2 producers might have originated from cattle through the use of cephalosporins such as ceftiofur and that cattle could be a reservoir of CTX-M-2-producing E. coli. Continuous and strategic surveillance of antimicrobial-resistant bacteria in livestock is essential to suppress further dissemination of these bacteria into society at large.
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U2 - 10.3201/eid1001.030219
DO - 10.3201/eid1001.030219
M3 - Article
C2 - 15078599
AN - SCOPUS:0347361563
SN - 1080-6040
VL - 10
SP - 69
EP - 75
JO - Emerging Infectious Diseases
JF - Emerging Infectious Diseases
IS - 1
ER -