抄録
Proper deposition and activation of Aurora B at the centromere is critical for faithful chromosome segregation in mammals. However, the mechanistic basis for abrupt Aurora B kinase activation at the centromere has not yet been fully understood. We demonstrate here that Aurora B-mediated phosphorylation of histone H2AX at serine 121 (H2AX-pS121) promotes Aurora B autophosphorylation and is essential for proper chromosome segregation. Aurora B-mediated H2AX-pS121 is specifically detected at the centromere during mitosis. H2AX depletion results in a severe defect in activation and deposition of Aurora B at this locus. A phosphomimic mutant of H2AX at S121 interacts with activated Aurora B more efficiently than wild-type in vitro. Taken together, these results propose a model in which Aurora B-mediated H2AX-pS121 probably provide a platform for Aurora B autoactivation circuitry at centromeres and thus play a pivotal role in proper chromosome segregation.
本文言語 | 英語 |
---|---|
論文番号 | 12059 |
ジャーナル | Nature communications |
巻 | 7 |
DOI | |
出版ステータス | 出版済み - 08-07-2016 |
外部発表 | はい |
All Science Journal Classification (ASJC) codes
- 化学一般
- 生化学、遺伝学、分子生物学一般
- 物理学および天文学一般