Establishment and characterization of four malignant pleural mesothelioma cell lines from Japanese patients

Noriyasu Usami, Takayuki Fukui, Masashi Kondo, Tetsuo Taniguchi, Toshihiko Yokoyama, Shoichi Mori, Kohei Yokoi, Yoshitsugu Horio, Kaoru Shimokata, Yoshitaka Sekido, Toyoaki Hida

研究成果: Article

97 引用 (Scopus)

抄録

Malignant pleural mesothelioma (MPM) is an asbestos-related malignancy that is highly resistant to current therapeutic modalities. We established four MPM cell lines (ACC-MESO-1, ACC-MESO-4, Y-MESO-8A and Y-MESO-8D) from Japanese patients, with the latter two from the same patient with biphasic-like characteristics of MPM, showing epithelial and sarcomatous phenotypes, respectively, in cell culture. These cells grew well in RPMI-1640 medium supplemented with 10% fetal bovine serum under 5% CO2. Mutation and expression analyses demonstrated that the tumor suppressor gene NF2, which is known to be one of the most frequently mutated in MPM, is mutated in ACCMESO- 1. We detected homozygous deletion of p16INK4A/p14ARF in all four MPM cell lines. However, mutations of other tumor suppressor genes, including TP53, and protooncogenes, including KRAS, NRAS, BRAF, EGFR and HER2, were not found in these cell lines. Polymerase chain reaction amplification of the simian virus 40 sequence did not detect any products. We also analyzed genetic alterations of six other MPM cell lines and confirmed frequent mutations of NF2 and p16INK4A/ p14ARF. To characterize the biological differences between Y-MESO-8A and Y-MESO-8D, we carried out cDNA microarray analysis and detected genes that were differentially expressed in these two cell lines. Thus, our new MPM cell lines seem to be useful as new models for studying various aspects of the biology of human MPM as well as materials for the development of future therapies.

元の言語English
ページ(範囲)387-394
ページ数8
ジャーナルCancer science
97
発行部数5
DOI
出版物ステータスPublished - 01-05-2006

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Cell Line
Tumor Suppressor Protein p14ARF
Tumor Suppressor Genes
Mutation
Simian virus 40
Malignant Mesothelioma
Asbestos
Microarray Analysis
Oligonucleotide Array Sequence Analysis
Cell Culture Techniques
Phenotype
Polymerase Chain Reaction
Therapeutics
Serum
Genes
Neoplasms

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

これを引用

Usami, Noriyasu ; Fukui, Takayuki ; Kondo, Masashi ; Taniguchi, Tetsuo ; Yokoyama, Toshihiko ; Mori, Shoichi ; Yokoi, Kohei ; Horio, Yoshitsugu ; Shimokata, Kaoru ; Sekido, Yoshitaka ; Hida, Toyoaki. / Establishment and characterization of four malignant pleural mesothelioma cell lines from Japanese patients. :: Cancer science. 2006 ; 巻 97, 番号 5. pp. 387-394.
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abstract = "Malignant pleural mesothelioma (MPM) is an asbestos-related malignancy that is highly resistant to current therapeutic modalities. We established four MPM cell lines (ACC-MESO-1, ACC-MESO-4, Y-MESO-8A and Y-MESO-8D) from Japanese patients, with the latter two from the same patient with biphasic-like characteristics of MPM, showing epithelial and sarcomatous phenotypes, respectively, in cell culture. These cells grew well in RPMI-1640 medium supplemented with 10{\%} fetal bovine serum under 5{\%} CO2. Mutation and expression analyses demonstrated that the tumor suppressor gene NF2, which is known to be one of the most frequently mutated in MPM, is mutated in ACCMESO- 1. We detected homozygous deletion of p16INK4A/p14ARF in all four MPM cell lines. However, mutations of other tumor suppressor genes, including TP53, and protooncogenes, including KRAS, NRAS, BRAF, EGFR and HER2, were not found in these cell lines. Polymerase chain reaction amplification of the simian virus 40 sequence did not detect any products. We also analyzed genetic alterations of six other MPM cell lines and confirmed frequent mutations of NF2 and p16INK4A/ p14ARF. To characterize the biological differences between Y-MESO-8A and Y-MESO-8D, we carried out cDNA microarray analysis and detected genes that were differentially expressed in these two cell lines. Thus, our new MPM cell lines seem to be useful as new models for studying various aspects of the biology of human MPM as well as materials for the development of future therapies.",
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Usami, N, Fukui, T, Kondo, M, Taniguchi, T, Yokoyama, T, Mori, S, Yokoi, K, Horio, Y, Shimokata, K, Sekido, Y & Hida, T 2006, 'Establishment and characterization of four malignant pleural mesothelioma cell lines from Japanese patients', Cancer science, 巻. 97, 番号 5, pp. 387-394. https://doi.org/10.1111/j.1349-7006.2006.00184.x

Establishment and characterization of four malignant pleural mesothelioma cell lines from Japanese patients. / Usami, Noriyasu; Fukui, Takayuki; Kondo, Masashi; Taniguchi, Tetsuo; Yokoyama, Toshihiko; Mori, Shoichi; Yokoi, Kohei; Horio, Yoshitsugu; Shimokata, Kaoru; Sekido, Yoshitaka; Hida, Toyoaki.

:: Cancer science, 巻 97, 番号 5, 01.05.2006, p. 387-394.

研究成果: Article

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T1 - Establishment and characterization of four malignant pleural mesothelioma cell lines from Japanese patients

AU - Usami, Noriyasu

AU - Fukui, Takayuki

AU - Kondo, Masashi

AU - Taniguchi, Tetsuo

AU - Yokoyama, Toshihiko

AU - Mori, Shoichi

AU - Yokoi, Kohei

AU - Horio, Yoshitsugu

AU - Shimokata, Kaoru

AU - Sekido, Yoshitaka

AU - Hida, Toyoaki

PY - 2006/5/1

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N2 - Malignant pleural mesothelioma (MPM) is an asbestos-related malignancy that is highly resistant to current therapeutic modalities. We established four MPM cell lines (ACC-MESO-1, ACC-MESO-4, Y-MESO-8A and Y-MESO-8D) from Japanese patients, with the latter two from the same patient with biphasic-like characteristics of MPM, showing epithelial and sarcomatous phenotypes, respectively, in cell culture. These cells grew well in RPMI-1640 medium supplemented with 10% fetal bovine serum under 5% CO2. Mutation and expression analyses demonstrated that the tumor suppressor gene NF2, which is known to be one of the most frequently mutated in MPM, is mutated in ACCMESO- 1. We detected homozygous deletion of p16INK4A/p14ARF in all four MPM cell lines. However, mutations of other tumor suppressor genes, including TP53, and protooncogenes, including KRAS, NRAS, BRAF, EGFR and HER2, were not found in these cell lines. Polymerase chain reaction amplification of the simian virus 40 sequence did not detect any products. We also analyzed genetic alterations of six other MPM cell lines and confirmed frequent mutations of NF2 and p16INK4A/ p14ARF. To characterize the biological differences between Y-MESO-8A and Y-MESO-8D, we carried out cDNA microarray analysis and detected genes that were differentially expressed in these two cell lines. Thus, our new MPM cell lines seem to be useful as new models for studying various aspects of the biology of human MPM as well as materials for the development of future therapies.

AB - Malignant pleural mesothelioma (MPM) is an asbestos-related malignancy that is highly resistant to current therapeutic modalities. We established four MPM cell lines (ACC-MESO-1, ACC-MESO-4, Y-MESO-8A and Y-MESO-8D) from Japanese patients, with the latter two from the same patient with biphasic-like characteristics of MPM, showing epithelial and sarcomatous phenotypes, respectively, in cell culture. These cells grew well in RPMI-1640 medium supplemented with 10% fetal bovine serum under 5% CO2. Mutation and expression analyses demonstrated that the tumor suppressor gene NF2, which is known to be one of the most frequently mutated in MPM, is mutated in ACCMESO- 1. We detected homozygous deletion of p16INK4A/p14ARF in all four MPM cell lines. However, mutations of other tumor suppressor genes, including TP53, and protooncogenes, including KRAS, NRAS, BRAF, EGFR and HER2, were not found in these cell lines. Polymerase chain reaction amplification of the simian virus 40 sequence did not detect any products. We also analyzed genetic alterations of six other MPM cell lines and confirmed frequent mutations of NF2 and p16INK4A/ p14ARF. To characterize the biological differences between Y-MESO-8A and Y-MESO-8D, we carried out cDNA microarray analysis and detected genes that were differentially expressed in these two cell lines. Thus, our new MPM cell lines seem to be useful as new models for studying various aspects of the biology of human MPM as well as materials for the development of future therapies.

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