Establishment of a new immortalized human corneal epithelial cell line (iHCE-NY1) for use in evaluating eye irritancy by in vitro test methods

Naoki Yamamoto, Yoshinao Kato, Atsushi Sato, Noriko Hiramatsu, Hiromi Yamashita, Mahito Ohkuma, Ei ichi Miyachi, Masayuki Horiguchi, Koji Hirano, Hajime Kojima

研究成果: Article

抄録

In vitro test methods that use human corneal epithelial cells to evaluate the eye irritation potency of chemical substances do not use human corneal epithelium because it has been difficult to maintain more than four passages. In this study, we make a new cell line comprising immortalized human corneal epithelial cells (iHCE-NY1). The IC50 of iHCE-NY1 cells is slightly higher than that of Statens Seruminstitut Rabbit Cornea (SIRC) cells, which are currently used in some in vitro test methods. CDKN1A in iHCE-NY1 cells was used as a marker of gene expression to indicate cell cycle activity. This enabled us to evaluate cell recovery characteristics at concentrations lower than the IC50 of cytotoxic tests.

元の言語English
ページ(範囲)742-748
ページ数7
ジャーナルIn Vitro Cellular and Developmental Biology - Animal
52
発行部数7
DOI
出版物ステータスPublished - 01-08-2016

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Epithelial Cells
Cell Line
Inhibitory Concentration 50
Corneal Epithelium
Cornea
Cell Cycle
Rabbits
Gene Expression
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • Developmental Biology
  • Cell Biology

これを引用

Yamamoto, Naoki ; Kato, Yoshinao ; Sato, Atsushi ; Hiramatsu, Noriko ; Yamashita, Hiromi ; Ohkuma, Mahito ; Miyachi, Ei ichi ; Horiguchi, Masayuki ; Hirano, Koji ; Kojima, Hajime. / Establishment of a new immortalized human corneal epithelial cell line (iHCE-NY1) for use in evaluating eye irritancy by in vitro test methods. :: In Vitro Cellular and Developmental Biology - Animal. 2016 ; 巻 52, 番号 7. pp. 742-748.
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abstract = "In vitro test methods that use human corneal epithelial cells to evaluate the eye irritation potency of chemical substances do not use human corneal epithelium because it has been difficult to maintain more than four passages. In this study, we make a new cell line comprising immortalized human corneal epithelial cells (iHCE-NY1). The IC50 of iHCE-NY1 cells is slightly higher than that of Statens Seruminstitut Rabbit Cornea (SIRC) cells, which are currently used in some in vitro test methods. CDKN1A in iHCE-NY1 cells was used as a marker of gene expression to indicate cell cycle activity. This enabled us to evaluate cell recovery characteristics at concentrations lower than the IC50 of cytotoxic tests.",
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Establishment of a new immortalized human corneal epithelial cell line (iHCE-NY1) for use in evaluating eye irritancy by in vitro test methods. / Yamamoto, Naoki; Kato, Yoshinao; Sato, Atsushi; Hiramatsu, Noriko; Yamashita, Hiromi; Ohkuma, Mahito; Miyachi, Ei ichi; Horiguchi, Masayuki; Hirano, Koji; Kojima, Hajime.

:: In Vitro Cellular and Developmental Biology - Animal, 巻 52, 番号 7, 01.08.2016, p. 742-748.

研究成果: Article

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AU - Yamashita, Hiromi

AU - Ohkuma, Mahito

AU - Miyachi, Ei ichi

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AU - Hirano, Koji

AU - Kojima, Hajime

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