Establishment of a new immortalized human corneal epithelial cell line (iHCE-NY1) for use in evaluating eye irritancy by in vitro test methods

Naoki Yamamoto; Yoshinao Kato; Atsushi Sato; Noriko Hiramatsu; Hiromi Yamashita; Mahito Ohkuma; Ei ichi Miyachi; Masayuki Horiguchi; Koji Hirano; Hajime Kojima

doi:10.1007/s11626-016-0038-9

Establishment of a new immortalized human corneal epithelial cell line (iHCE-NY1) for use in evaluating eye irritancy by in vitro test methods

Naoki Yamamoto
, Yoshinao Kato
, Atsushi Sato
, Noriko Hiramatsu
, Hiromi Yamashita
, Mahito Ohkuma
, Ei ichi Miyachi
, Masayuki Horiguchi
, Koji Hirano
, Hajime Kojima

第１・眼科

研究成果: ジャーナルへの寄稿 › 学術論文 › 査読

9 被引用数 (Scopus)

抄録

In vitro test methods that use human corneal epithelial cells to evaluate the eye irritation potency of chemical substances do not use human corneal epithelium because it has been difficult to maintain more than four passages. In this study, we make a new cell line comprising immortalized human corneal epithelial cells (iHCE-NY1). The IC50 of iHCE-NY1 cells is slightly higher than that of Statens Seruminstitut Rabbit Cornea (SIRC) cells, which are currently used in some in vitro test methods. CDKN1A in iHCE-NY1 cells was used as a marker of gene expression to indicate cell cycle activity. This enabled us to evaluate cell recovery characteristics at concentrations lower than the IC50 of cytotoxic tests.

本文言語	英語
ページ（範囲）	742-748
ページ数	7
ジャーナル	In Vitro Cellular and Developmental Biology - Animal
巻	52
号	7
DOI	https://doi.org/10.1007/s11626-016-0038-9
出版ステータス	出版済み - 01-08-2016

All Science Journal Classification (ASJC) codes

発生生物学
細胞生物学

文献へのアクセス

10.1007/s11626-016-0038-9

他のファイルとリンク

フィンガープリント

「Establishment of a new immortalized human corneal epithelial cell line (iHCE-NY1) for use in evaluating eye irritancy by in vitro test methods」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル

Yamamoto, N., Kato, Y., Sato, A., Hiramatsu, N., Yamashita, H., Ohkuma, M., Miyachi, E. I., Horiguchi, M., Hirano, K., & Kojima, H. (2016). Establishment of a new immortalized human corneal epithelial cell line (iHCE-NY1) for use in evaluating eye irritancy by in vitro test methods. In Vitro Cellular and Developmental Biology - Animal, 52(7), 742-748. https://doi.org/10.1007/s11626-016-0038-9

@article{4c6055f0bdce42a2b25ef8426e922ad0,

title = "Establishment of a new immortalized human corneal epithelial cell line (iHCE-NY1) for use in evaluating eye irritancy by in vitro test methods",

abstract = "In vitro test methods that use human corneal epithelial cells to evaluate the eye irritation potency of chemical substances do not use human corneal epithelium because it has been difficult to maintain more than four passages. In this study, we make a new cell line comprising immortalized human corneal epithelial cells (iHCE-NY1). The IC50 of iHCE-NY1 cells is slightly higher than that of Statens Seruminstitut Rabbit Cornea (SIRC) cells, which are currently used in some in vitro test methods. CDKN1A in iHCE-NY1 cells was used as a marker of gene expression to indicate cell cycle activity. This enabled us to evaluate cell recovery characteristics at concentrations lower than the IC50 of cytotoxic tests.",

author = "Naoki Yamamoto and Yoshinao Kato and Atsushi Sato and Noriko Hiramatsu and Hiromi Yamashita and Mahito Ohkuma and Miyachi, \{Ei ichi\} and Masayuki Horiguchi and Koji Hirano and Hajime Kojima",

note = "Publisher Copyright: {\textcopyright} 2016, The Society for In Vitro Biology.",

year = "2016",

month = aug,

day = "1",

doi = "10.1007/s11626-016-0038-9",

language = "English",

volume = "52",

pages = "742--748",

journal = "In Vitro Cellular and Developmental Biology - Animal",

issn = "1071-2690",

publisher = "Springer",

number = "7",

}

Yamamoto, N, Kato, Y, Sato, A, Hiramatsu, N, Yamashita, H, Ohkuma, M, Miyachi, EI, Horiguchi, M, Hirano, K & Kojima, H 2016, 'Establishment of a new immortalized human corneal epithelial cell line (iHCE-NY1) for use in evaluating eye irritancy by in vitro test methods', In Vitro Cellular and Developmental Biology - Animal, vol. 52, no. 7, pp. 742-748. https://doi.org/10.1007/s11626-016-0038-9

Establishment of a new immortalized human corneal epithelial cell line (iHCE-NY1) for use in evaluating eye irritancy by in vitro test methods. / Yamamoto, Naoki; Kato, Yoshinao; Sato, Atsushi その他.
In: In Vitro Cellular and Developmental Biology - Animal, Vol. 52, No. 7, 01.08.2016, p. 742-748.

研究成果: ジャーナルへの寄稿 › 学術論文 › 査読

TY - JOUR

T1 - Establishment of a new immortalized human corneal epithelial cell line (iHCE-NY1) for use in evaluating eye irritancy by in vitro test methods

AU - Yamamoto, Naoki

AU - Kato, Yoshinao

AU - Sato, Atsushi

AU - Hiramatsu, Noriko

AU - Yamashita, Hiromi

AU - Ohkuma, Mahito

AU - Miyachi, Ei ichi

AU - Horiguchi, Masayuki

AU - Hirano, Koji

AU - Kojima, Hajime

PY - 2016/8/1

Y1 - 2016/8/1

N2 - In vitro test methods that use human corneal epithelial cells to evaluate the eye irritation potency of chemical substances do not use human corneal epithelium because it has been difficult to maintain more than four passages. In this study, we make a new cell line comprising immortalized human corneal epithelial cells (iHCE-NY1). The IC50 of iHCE-NY1 cells is slightly higher than that of Statens Seruminstitut Rabbit Cornea (SIRC) cells, which are currently used in some in vitro test methods. CDKN1A in iHCE-NY1 cells was used as a marker of gene expression to indicate cell cycle activity. This enabled us to evaluate cell recovery characteristics at concentrations lower than the IC50 of cytotoxic tests.

AB - In vitro test methods that use human corneal epithelial cells to evaluate the eye irritation potency of chemical substances do not use human corneal epithelium because it has been difficult to maintain more than four passages. In this study, we make a new cell line comprising immortalized human corneal epithelial cells (iHCE-NY1). The IC50 of iHCE-NY1 cells is slightly higher than that of Statens Seruminstitut Rabbit Cornea (SIRC) cells, which are currently used in some in vitro test methods. CDKN1A in iHCE-NY1 cells was used as a marker of gene expression to indicate cell cycle activity. This enabled us to evaluate cell recovery characteristics at concentrations lower than the IC50 of cytotoxic tests.

UR - https://www.scopus.com/pages/publications/84964582957

UR - https://www.scopus.com/pages/publications/84964582957#tab=citedBy

U2 - 10.1007/s11626-016-0038-9

DO - 10.1007/s11626-016-0038-9

M3 - Article

C2 - 27130679

AN - SCOPUS:84964582957

SN - 1071-2690

VL - 52

SP - 742

EP - 748

JO - In Vitro Cellular and Developmental Biology - Animal

JF - In Vitro Cellular and Developmental Biology - Animal

IS - 7

ER -