Establishment of CYP2D6 reference samples by multiple validated genotyping platforms

H. Fang, X. Liu, J. Ramírez, N. Choudhury, M. Kubo, H. K. Im, A. Konkashbaev, N. J. Cox, M. J. Ratain, Y. Nakamura, P. H. O'Donnell

研究成果: Article査読

20 被引用数 (Scopus)

抄録

Cytochrome P450 2D6 (cytochrome P450, family 2, subfamily D, polypeptide 6 (CYP2D6)), a highly polymorphic drug-metabolizing enzyme, is involved in the metabolism of one-quarter of the most commonly prescribed medications. Here we have applied multiple genotyping methods and Sanger sequencing to assign precise and reproducible CYP2D6 genotypes, including copy numbers, for 48 HapMap samples. Furthermore, by analyzing a set of 50 human liver microsomes using endoxifen formation from N-desmethyl-tamoxifen as the phenotype of interest, we observed a significant positive correlation between CYP2D6 genotype-assigned activity score and endoxifen formation rate (rs =0.68 by rank correlation test, P=5.3 × 10-8), which corroborated the genotype-phenotype prediction derived from our genotyping methodologies. In the future, these 48 publicly available HapMap samples characterized by multiple substantiated CYP2D6 genotyping platforms could serve as a reference resource for assay development, validation, quality control and proficiency testing for other CYP2D6 genotyping projects and for programs pursuing clinical pharmacogenomic testing implementation.

本文言語English
ページ(範囲)564-572
ページ数9
ジャーナルPharmacogenomics Journal
14
6
DOI
出版ステータスPublished - 11-12-2014
外部発表はい

All Science Journal Classification (ASJC) codes

  • 分子医療
  • 遺伝学
  • 薬理学

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