Establishment of the culture model system that reflects the process of terminal differentiation of connective tissue-type mast cells

Hirotsugu Takano; Shunsuke Nakazawa; Yasushi Okuno; Naritoshi Shirata; Sohken Tsuchiya; Takayuki Kainoh; Seigoh Takamatsu; Kazuyuki Furuta; Yoshitaka Taketomi; Yuko Naito; Hiromu Takematsu; Yasunori Kozutsumi; Gozoh Tsujimoto; Makoto Murakami; Ichiro Kudo; Atsushi Ichikawa; Kazuhisa Nakayama; Yukihiko Sugimoto; Satoshi Tanaka

doi:10.1016/j.febslet.2008.03.033

Establishment of the culture model system that reflects the process of terminal differentiation of connective tissue-type mast cells

Hirotsugu Takano, Shunsuke Nakazawa, Yasushi Okuno, Naritoshi Shirata, Sohken Tsuchiya, Takayuki Kainoh, Seigoh Takamatsu, Kazuyuki Furuta, Yoshitaka Taketomi, Yuko Naito, Hiromu Takematsu, Yasunori Kozutsumi, Gozoh Tsujimoto, Makoto Murakami, Ichiro Kudo, Atsushi Ichikawa, Kazuhisa Nakayama, Yukihiko Sugimoto, Satoshi Tanaka

免疫医科学

研究成果: ジャーナルへの寄稿 › 学術論文 › 査読

40 被引用数 (Scopus)

抄録

To understand physiological roles of tissue mast cells, we established a culture system where bone marrow-derived immature mast cells differentiate into the connective tissue-type mast cell (CTMC)-like cells through modifying the previous co-culture system with Swiss 3T3 fibroblasts. Our system was found to reproducibly mimic the differentiation of CTMCs on the basis of several criteria, such as granule maturation and sensitivity to cationic secretagogues. The gene expression profile obtained by the microarray analyses was found to reflect many aspects of the differentiation. Our system is thus helpful to gain deeper insights into terminal differentiation of CTMCs.

本文言語	英語
ページ（範囲）	1444-1450
ページ数	7
ジャーナル	FEBS Letters
巻	582
号	10
DOI	https://doi.org/10.1016/j.febslet.2008.03.033
出版ステータス	出版済み - 30-04-2008

All Science Journal Classification (ASJC) codes

生物理学
構造生物学
生化学
分子生物学
遺伝学
細胞生物学

文献へのアクセス

10.1016/j.febslet.2008.03.033

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引用スタイル

Takano, H., Nakazawa, S., Okuno, Y., Shirata, N., Tsuchiya, S., Kainoh, T., Takamatsu, S., Furuta, K., Taketomi, Y., Naito, Y., Takematsu, H., Kozutsumi, Y., Tsujimoto, G., Murakami, M., Kudo, I., Ichikawa, A., Nakayama, K., Sugimoto, Y., & Tanaka, S. (2008). Establishment of the culture model system that reflects the process of terminal differentiation of connective tissue-type mast cells. FEBS Letters, 582(10), 1444-1450. https://doi.org/10.1016/j.febslet.2008.03.033

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title = "Establishment of the culture model system that reflects the process of terminal differentiation of connective tissue-type mast cells",

abstract = "To understand physiological roles of tissue mast cells, we established a culture system where bone marrow-derived immature mast cells differentiate into the connective tissue-type mast cell (CTMC)-like cells through modifying the previous co-culture system with Swiss 3T3 fibroblasts. Our system was found to reproducibly mimic the differentiation of CTMCs on the basis of several criteria, such as granule maturation and sensitivity to cationic secretagogues. The gene expression profile obtained by the microarray analyses was found to reflect many aspects of the differentiation. Our system is thus helpful to gain deeper insights into terminal differentiation of CTMCs.",

keywords = "Differentiation, G, Gene expression, Mast cell",

author = "Hirotsugu Takano and Shunsuke Nakazawa and Yasushi Okuno and Naritoshi Shirata and Sohken Tsuchiya and Takayuki Kainoh and Seigoh Takamatsu and Kazuyuki Furuta and Yoshitaka Taketomi and Yuko Naito and Hiromu Takematsu and Yasunori Kozutsumi and Gozoh Tsujimoto and Makoto Murakami and Ichiro Kudo and Atsushi Ichikawa and Kazuhisa Nakayama and Yukihiko Sugimoto and Satoshi Tanaka",

note = "Funding Information: This study was supported in part by 21st Century COE Program “Knowledge Information Infrastructure for Genome Science”, a grant from Uehara Memorial Foundation, Naito Foundation, Kao Foundation for Arts and Sciences, Sankyo Foundation of Life Science, and by Grants-in-Aid for Scientific Research from the Ministry of Education, Culture, Science, Sports and Technology of Japan and from the Ministry of Health and Labor of Japan. ",

year = "2008",

month = apr,

day = "30",

doi = "10.1016/j.febslet.2008.03.033",

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Takano, H, Nakazawa, S, Okuno, Y, Shirata, N, Tsuchiya, S, Kainoh, T, Takamatsu, S, Furuta, K, Taketomi, Y, Naito, Y, Takematsu, H, Kozutsumi, Y, Tsujimoto, G, Murakami, M, Kudo, I, Ichikawa, A, Nakayama, K, Sugimoto, Y & Tanaka, S 2008, 'Establishment of the culture model system that reflects the process of terminal differentiation of connective tissue-type mast cells', FEBS Letters, vol. 582, no. 10, pp. 1444-1450. https://doi.org/10.1016/j.febslet.2008.03.033

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T1 - Establishment of the culture model system that reflects the process of terminal differentiation of connective tissue-type mast cells

AU - Takano, Hirotsugu

AU - Nakazawa, Shunsuke

AU - Okuno, Yasushi

AU - Shirata, Naritoshi

AU - Tsuchiya, Sohken

AU - Kainoh, Takayuki

AU - Takamatsu, Seigoh

AU - Furuta, Kazuyuki

AU - Taketomi, Yoshitaka

AU - Naito, Yuko

AU - Takematsu, Hiromu

AU - Kozutsumi, Yasunori

AU - Tsujimoto, Gozoh

AU - Murakami, Makoto

AU - Kudo, Ichiro

AU - Ichikawa, Atsushi

AU - Nakayama, Kazuhisa

AU - Sugimoto, Yukihiko

AU - Tanaka, Satoshi

N1 - Funding Information: This study was supported in part by 21st Century COE Program “Knowledge Information Infrastructure for Genome Science”, a grant from Uehara Memorial Foundation, Naito Foundation, Kao Foundation for Arts and Sciences, Sankyo Foundation of Life Science, and by Grants-in-Aid for Scientific Research from the Ministry of Education, Culture, Science, Sports and Technology of Japan and from the Ministry of Health and Labor of Japan.

PY - 2008/4/30

Y1 - 2008/4/30

N2 - To understand physiological roles of tissue mast cells, we established a culture system where bone marrow-derived immature mast cells differentiate into the connective tissue-type mast cell (CTMC)-like cells through modifying the previous co-culture system with Swiss 3T3 fibroblasts. Our system was found to reproducibly mimic the differentiation of CTMCs on the basis of several criteria, such as granule maturation and sensitivity to cationic secretagogues. The gene expression profile obtained by the microarray analyses was found to reflect many aspects of the differentiation. Our system is thus helpful to gain deeper insights into terminal differentiation of CTMCs.

AB - To understand physiological roles of tissue mast cells, we established a culture system where bone marrow-derived immature mast cells differentiate into the connective tissue-type mast cell (CTMC)-like cells through modifying the previous co-culture system with Swiss 3T3 fibroblasts. Our system was found to reproducibly mimic the differentiation of CTMCs on the basis of several criteria, such as granule maturation and sensitivity to cationic secretagogues. The gene expression profile obtained by the microarray analyses was found to reflect many aspects of the differentiation. Our system is thus helpful to gain deeper insights into terminal differentiation of CTMCs.

KW - Differentiation

KW - G

KW - Gene expression

KW - Mast cell

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