Establishment of the culture model system that reflects the process of terminal differentiation of connective tissue-type mast cells

Hirotsugu Takano, Shunsuke Nakazawa, Yasushi Okuno, Naritoshi Shirata, Sohken Tsuchiya, Takayuki Kainoh, Seigoh Takamatsu, Kazuyuki Furuta, Yoshitaka Taketomi, Yuko Naito, Hiromu Takematsu, Yasunori Kozutsumi, Gozoh Tsujimoto, Makoto Murakami, Ichiro Kudo, Atsushi Ichikawa, Kazuhisa Nakayama, Yukihiko Sugimoto, Satoshi Tanaka

研究成果: Article査読

31 被引用数 (Scopus)

抄録

To understand physiological roles of tissue mast cells, we established a culture system where bone marrow-derived immature mast cells differentiate into the connective tissue-type mast cell (CTMC)-like cells through modifying the previous co-culture system with Swiss 3T3 fibroblasts. Our system was found to reproducibly mimic the differentiation of CTMCs on the basis of several criteria, such as granule maturation and sensitivity to cationic secretagogues. The gene expression profile obtained by the microarray analyses was found to reflect many aspects of the differentiation. Our system is thus helpful to gain deeper insights into terminal differentiation of CTMCs.

本文言語English
ページ(範囲)1444-1450
ページ数7
ジャーナルFEBS Letters
582
10
DOI
出版ステータスPublished - 30-04-2008
外部発表はい

All Science Journal Classification (ASJC) codes

  • 生物理学
  • 構造生物学
  • 生化学
  • 分子生物学
  • 遺伝学
  • 細胞生物学

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