Exogenous nitric oxide enhances the synthesis of type I collagen and heat shock protein 47 by normal human dermal fibroblasts

Kei Obayashi, Hirohiko Akamatsu, Yuri Okano, Kayoko Matsunaga, Hitoshi Masaki

研究成果: Article査読

39 被引用数 (Scopus)

抄録

Background: It is well established that the alterations of dermal matrix contributes to skin aging characterized by wrinkles. On the other hand, physiological NO is useful to maintain skin homeostasis such as a vasodilatation. However, a role of NO on production of dermal matrix has been clarified. Objective: In this study, we have attempted to analyze the role of NO on type I collagen synthesis of normal human dermal fibroblasts including expression of procollagen αI S(1) mRNA/protein and heat shock protein 47 (HSP47). Methods: The effects of NO which was generated by two types of NO donors, sodium nitroprusside (SNP) and S-nitroso-N-acetylpenicillamine (SNAP), on type I collagen and HSP47 and their related mRNA expression were examined with ELISA and RT-PCR. Results: NO was significantly accelerated the production of type I collagen by fibroblasts corresponding with up-regulation of procollagen αI (1) mRNA. Furthermore, NO increased both levels of HSP47 protein and mRNA in fibroblasts in a dose-dependent manner. Conclusions: These results suggest that NO has dual effects on collagen synthesis by fibroblasts as follows; one is the direct stimulation of collagen synthesis due to the up-regulation of procollagen αI(1) mRNA, and the other is an indirect effect through the increase of HSP47 mRNA expression. This is the first report that exogenous NO stimulates HSP47 production by dermal fibroblasts.

本文言語English
ページ(範囲)121-126
ページ数6
ジャーナルJournal of Dermatological Science
41
2
DOI
出版ステータスPublished - 02-2006
外部発表はい

All Science Journal Classification (ASJC) codes

  • 生化学
  • 分子生物学
  • 皮膚病学

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