@article{b4e413fa9d7947fba4a0fcbe03a8b02f,
title = "Expanding the Repertoire of Optogenetically Targeted Cells with an Enhanced Gene Expression System",
abstract = "Drosophila neuroblasts (NBs) have emerged as a model for stem cell biology that is ideal for genetic analysis but is limited by the lack of cell-type-specific gene expression data. Here, we describe a method for isolating large numbers of pure NBs and differentiating neurons that retain both cell-cycle and lineage characteristics. We determine transcriptional profiles by mRNA sequencing and identify 28 predicted NB-specific transcription factors that can be arranged in a network containing hubs for Notch signaling, growth control, and chromatin regulation. Overexpression and RNA interference for these factors identify Klumpfuss as a regulator of self-renewal. We show that loss of Klumpfuss function causes premature differentiation and that overexpression results in the formation of transplantable brain tumors. Our data represent a valuable resource for investigating Drosophila developmental neurobiology, and the described method can be applied to other invertebrate stem cell lineages as well.",
author = "Tanaka, {Kenji F.} and Ko Matsui and Takuya Sasaki and Hiromi Sano and Shouta Sugio and Kai Fan and Ren{\'e} Hen and Junichi Nakai and Yuchio Yanagawa and Hidetoshi Hasuwa and Masaru Okabe and Karl Deisseroth and Kazuhiro Ikenaka and Akihiro Yamanaka",
note = "Funding Information: This work was supported by grants from the Takeda Science Foundation to K.F.T. and A.Y., a Grant-in-Aid for Young Scientists (A) from the Ministry of Education, Culture, Sports, Science and Technology of Japan (MEXT) to K.F.T. [23680042], a Grant-in-Aid for Scientific Research on Innovative Areas “Mesoscopic Neurocircuitry” from MEXT to K.M. [23115521], Y.Y. [23115503], and A.Y. [23115519], a Grant-in-Aid for Scientific Research on Innovative Areas “Brain Environment” from MEXT to K.F.T. [24111551], a Grant-in-Aid for Challenging Exploratory Research from MEXT to K.F.T. [24650219], a Grant-in-Aid for Scientific Research (C) from MEXT to K.M. [22500362], a Grant-in-Aid for Scientific Research (B) from MEXT to Y.Y. [22300105] and A.Y. [23300142], and PRESTO from Japan Science and Technology Agency (JST) to A.Y. We thank Yoko Esaki and Kiyo Kawata for technical assistance with producing transgenic mouse lines. ",
year = "2012",
month = aug,
day = "30",
doi = "10.1016/j.celrep.2012.06.011",
language = "English",
volume = "2",
pages = "397--406",
journal = "Cell Reports",
issn = "2211-1247",
publisher = "Cell Press",
number = "2",
}