Expression analysis of a mouse orthologue of HSFY, a candidate for the azoospermic factor on the human Y chromosome

Keigo Kinoshita, Toshikatsu Shinka, Youichi Sato, Hiroki Kurahashi, Hiroe Kowa, Gang Chen, Mayumi Umeno, Kazunori Toida, Emi Kiyokage, Takuro Nakano, Susumu Ito, Yutaka Nakahori

研究成果: Article

14 引用 (Scopus)

抄録

Heat shock transcription factor on Y (HSFY) is located in one of three candidate regions for azoospermic factor (AZF), AZFb on the Y chromosome. We and others have already revealed that some azoospermic males are missing the regions of the Y chromosome including HSFY. Previously, we showed that murine HSFY-like sequence (mHSFYL (Riken cDNA 4933413G11Rik)), which is the mouse orthologue of HSFY, is exclusively expressed in testis. The sequences encoding the presumed DNA-binding domain in HSFY and mHSFYL were found in other mammals such as dogs, cows and chickens. To elucidate mHSFYL expression in the testes in detail, we carried out in situ hybridization. mHSFYL was predominantly expressed in round spermatids. Furthermore, we clarified the intracellular distribution of mHSFYL in COS1 cells with HA-or GFP-tagged proteins. Both HA-mHSFYL and GFP-mHSFYL were located in the nucleus. Our results suggest that HSFY/mHSFYL may have evolutionarily conserved functions for spermatogenesis.

元の言語English
ページ(範囲)117-122
ページ数6
ジャーナルJournal of Medical Investigation
53
発行部数1-2
DOI
出版物ステータスPublished - 01-02-2006

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Chromosomes, Human, Y
Chromosomes
Y Chromosome
Testis
Mammals
Spermatids
Spermatogenesis
In Situ Hybridization
heat shock transcription factor
Chickens
Complementary DNA
Dogs
DNA

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

これを引用

Kinoshita, Keigo ; Shinka, Toshikatsu ; Sato, Youichi ; Kurahashi, Hiroki ; Kowa, Hiroe ; Chen, Gang ; Umeno, Mayumi ; Toida, Kazunori ; Kiyokage, Emi ; Nakano, Takuro ; Ito, Susumu ; Nakahori, Yutaka. / Expression analysis of a mouse orthologue of HSFY, a candidate for the azoospermic factor on the human Y chromosome. :: Journal of Medical Investigation. 2006 ; 巻 53, 番号 1-2. pp. 117-122.
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abstract = "Heat shock transcription factor on Y (HSFY) is located in one of three candidate regions for azoospermic factor (AZF), AZFb on the Y chromosome. We and others have already revealed that some azoospermic males are missing the regions of the Y chromosome including HSFY. Previously, we showed that murine HSFY-like sequence (mHSFYL (Riken cDNA 4933413G11Rik)), which is the mouse orthologue of HSFY, is exclusively expressed in testis. The sequences encoding the presumed DNA-binding domain in HSFY and mHSFYL were found in other mammals such as dogs, cows and chickens. To elucidate mHSFYL expression in the testes in detail, we carried out in situ hybridization. mHSFYL was predominantly expressed in round spermatids. Furthermore, we clarified the intracellular distribution of mHSFYL in COS1 cells with HA-or GFP-tagged proteins. Both HA-mHSFYL and GFP-mHSFYL were located in the nucleus. Our results suggest that HSFY/mHSFYL may have evolutionarily conserved functions for spermatogenesis.",
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Kinoshita, K, Shinka, T, Sato, Y, Kurahashi, H, Kowa, H, Chen, G, Umeno, M, Toida, K, Kiyokage, E, Nakano, T, Ito, S & Nakahori, Y 2006, 'Expression analysis of a mouse orthologue of HSFY, a candidate for the azoospermic factor on the human Y chromosome', Journal of Medical Investigation, 巻. 53, 番号 1-2, pp. 117-122. https://doi.org/10.2152/jmi.53.117

Expression analysis of a mouse orthologue of HSFY, a candidate for the azoospermic factor on the human Y chromosome. / Kinoshita, Keigo; Shinka, Toshikatsu; Sato, Youichi; Kurahashi, Hiroki; Kowa, Hiroe; Chen, Gang; Umeno, Mayumi; Toida, Kazunori; Kiyokage, Emi; Nakano, Takuro; Ito, Susumu; Nakahori, Yutaka.

:: Journal of Medical Investigation, 巻 53, 番号 1-2, 01.02.2006, p. 117-122.

研究成果: Article

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AU - Kinoshita, Keigo

AU - Shinka, Toshikatsu

AU - Sato, Youichi

AU - Kurahashi, Hiroki

AU - Kowa, Hiroe

AU - Chen, Gang

AU - Umeno, Mayumi

AU - Toida, Kazunori

AU - Kiyokage, Emi

AU - Nakano, Takuro

AU - Ito, Susumu

AU - Nakahori, Yutaka

PY - 2006/2/1

Y1 - 2006/2/1

N2 - Heat shock transcription factor on Y (HSFY) is located in one of three candidate regions for azoospermic factor (AZF), AZFb on the Y chromosome. We and others have already revealed that some azoospermic males are missing the regions of the Y chromosome including HSFY. Previously, we showed that murine HSFY-like sequence (mHSFYL (Riken cDNA 4933413G11Rik)), which is the mouse orthologue of HSFY, is exclusively expressed in testis. The sequences encoding the presumed DNA-binding domain in HSFY and mHSFYL were found in other mammals such as dogs, cows and chickens. To elucidate mHSFYL expression in the testes in detail, we carried out in situ hybridization. mHSFYL was predominantly expressed in round spermatids. Furthermore, we clarified the intracellular distribution of mHSFYL in COS1 cells with HA-or GFP-tagged proteins. Both HA-mHSFYL and GFP-mHSFYL were located in the nucleus. Our results suggest that HSFY/mHSFYL may have evolutionarily conserved functions for spermatogenesis.

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