Expression analysis of a mouse orthologue of HSFY, a candidate for the azoospermic factor on the human Y chromosome

Keigo Kinoshita, Toshikatsu Shinka, Youichi Sato, Hiroki Kurahashi, Hiroe Kowa, Gang Chen, Mayumi Umeno, Kazunori Toida, Emi Kiyokage, Takuro Nakano, Susumu Ito, Yutaka Nakahori

研究成果: Article

15 引用 (Scopus)

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Heat shock transcription factor on Y (HSFY) is located in one of three candidate regions for azoospermic factor (AZF), AZFb on the Y chromosome. We and others have already revealed that some azoospermic males are missing the regions of the Y chromosome including HSFY. Previously, we showed that murine HSFY-like sequence (mHSFYL (Riken cDNA 4933413G11Rik)), which is the mouse orthologue of HSFY, is exclusively expressed in testis. The sequences encoding the presumed DNA-binding domain in HSFY and mHSFYL were found in other mammals such as dogs, cows and chickens. To elucidate mHSFYL expression in the testes in detail, we carried out in situ hybridization. mHSFYL was predominantly expressed in round spermatids. Furthermore, we clarified the intracellular distribution of mHSFYL in COS1 cells with HA-or GFP-tagged proteins. Both HA-mHSFYL and GFP-mHSFYL were located in the nucleus. Our results suggest that HSFY/mHSFYL may have evolutionarily conserved functions for spermatogenesis.

元の言語English
ページ(範囲)117-122
ページ数6
ジャーナルJournal of Medical Investigation
53
発行部数1-2
DOI
出版物ステータスPublished - 01-02-2006

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

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    Kinoshita, K., Shinka, T., Sato, Y., Kurahashi, H., Kowa, H., Chen, G., Umeno, M., Toida, K., Kiyokage, E., Nakano, T., Ito, S., & Nakahori, Y. (2006). Expression analysis of a mouse orthologue of HSFY, a candidate for the azoospermic factor on the human Y chromosome. Journal of Medical Investigation, 53(1-2), 117-122. https://doi.org/10.2152/jmi.53.117