Expression analysis of gibberellin-regulated protein in peach by reverse transcription-quantitative PCR

Natsuki Kawai; Yuki Hirakawa; Shinya Matsumoto; Akihiro Itai; Kayoko Matsunaga; Hiroshi Narita; Keiko Momma

doi:10.1093/bbb/zbac123

Expression analysis of gibberellin-regulated protein in peach by reverse transcription-quantitative PCR

Natsuki Kawai
, Yuki Hirakawa
, Shinya Matsumoto
, Akihiro Itai
, Kayoko Matsunaga
, Hiroshi Narita
, Keiko Momma

医学部・医学科

研究成果: ジャーナルへの寄稿 › 学術論文 › 査読

1 被引用数 (Scopus)

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Gibberellin-regulated protein (GRP) is a fruit severe allergen. The amounts of GRP expression normalized against actin in peach were determined by reverse transcription-quantitative PCR (RT-qPCR). The results were consistent with those determined by enzyme-linked immunosorbent assay (ELISA). The GRP expression was more evident in flesh than peel and increased rapidly in the maturing period. This approach is applicable to estimate the amount of GRP in other plants.

本文言語	英語
ページ（範囲）	1459-1461
ページ数	3
ジャーナル	Bioscience, Biotechnology and Biochemistry
巻	86
号	10
DOI	https://doi.org/10.1093/bbb/zbac123
出版ステータス	出版済み - 01-10-2022

All Science Journal Classification (ASJC) codes

医学一般

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10.1093/bbb/zbac123

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title = "Expression analysis of gibberellin-regulated protein in peach by reverse transcription-quantitative PCR",

abstract = "Gibberellin-regulated protein (GRP) is a fruit severe allergen. The amounts of GRP expression normalized against actin in peach were determined by reverse transcription-quantitative PCR (RT-qPCR). The results were consistent with those determined by enzyme-linked immunosorbent assay (ELISA). The GRP expression was more evident in flesh than peel and increased rapidly in the maturing period. This approach is applicable to estimate the amount of GRP in other plants.",

keywords = "GRP, RT-qPCR, component-resolved diagnosis, fruits allergy, peach",

author = "Natsuki Kawai and Yuki Hirakawa and Shinya Matsumoto and Akihiro Itai and Kayoko Matsunaga and Hiroshi Narita and Keiko Momma",

note = "Publisher Copyright: {\textcopyright} 2022 The Author(s). Published by Oxford University Press on behalf of Japan Society for Bioscience, Biotechnology, and Agrochemistry.",

year = "2022",

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day = "1",

doi = "10.1093/bbb/zbac123",

language = "English",

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journal = "Bioscience, Biotechnology and Biochemistry",

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T1 - Expression analysis of gibberellin-regulated protein in peach by reverse transcription-quantitative PCR

AU - Kawai, Natsuki

AU - Hirakawa, Yuki

AU - Matsumoto, Shinya

AU - Itai, Akihiro

AU - Matsunaga, Kayoko

AU - Narita, Hiroshi

AU - Momma, Keiko

PY - 2022/10/1

Y1 - 2022/10/1

N2 - Gibberellin-regulated protein (GRP) is a fruit severe allergen. The amounts of GRP expression normalized against actin in peach were determined by reverse transcription-quantitative PCR (RT-qPCR). The results were consistent with those determined by enzyme-linked immunosorbent assay (ELISA). The GRP expression was more evident in flesh than peel and increased rapidly in the maturing period. This approach is applicable to estimate the amount of GRP in other plants.

AB - Gibberellin-regulated protein (GRP) is a fruit severe allergen. The amounts of GRP expression normalized against actin in peach were determined by reverse transcription-quantitative PCR (RT-qPCR). The results were consistent with those determined by enzyme-linked immunosorbent assay (ELISA). The GRP expression was more evident in flesh than peel and increased rapidly in the maturing period. This approach is applicable to estimate the amount of GRP in other plants.

KW - GRP

KW - RT-qPCR

KW - component-resolved diagnosis

KW - fruits allergy

KW - peach

UR - https://www.scopus.com/pages/publications/85138493576

UR - https://www.scopus.com/pages/publications/85138493576#tab=citedBy

U2 - 10.1093/bbb/zbac123

DO - 10.1093/bbb/zbac123

M3 - Article

C2 - 35867877

AN - SCOPUS:85138493576

SN - 0916-8451

VL - 86

SP - 1459

EP - 1461

JO - Bioscience, Biotechnology and Biochemistry

JF - Bioscience, Biotechnology and Biochemistry

IS - 10

ER -

Expression analysis of gibberellin-regulated protein in peach by reverse transcription-quantitative PCR

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