TY - JOUR
T1 - Expression and localization of aging markers in lacrimal gland of chronic graft-versus-host disease
AU - Kawai, Masataka
AU - Ogawa, Yoko
AU - Shimmura, Shigeto
AU - Ohta, Shigeki
AU - Suzuki, Takanori
AU - Kawamura, Naoshi
AU - Kuwana, Masataka
AU - Kawakami, Yutaka
AU - Tsubota, Kazuo
N1 - Funding Information:
This work was supported by the Japanese Ministry of Education, Science, Sports and Culture, #23592590. We thank Prof. Shinichiro Okamoto, and Prof. Hideyuki Saya for valuable discussion. We thank Miss Mai Tadaki, Dr. Toshihiro Nagai, Dr. Shigeru Nakamura and Dr. Toshihiro Imada, Keio University School of Medicine, for excellent technical assistance. We also thank Mr. Kenji Morii, and Dr. Hajime Kamijuku Keio University School of Medicine for expert technical advices.
PY - 2013/8/29
Y1 - 2013/8/29
N2 - Aging is commonly defined as the accumulation of diverse deleterious changes in cells and tissues with advancing age. To investigate whether aging changes are involved in the lacrimal glands of chronic graft-versus-host disease (cGVHD) model mice, we obtained the specimens from cGVHD model mice, untreated aged and young mice, and examined by histopathology, and immunoblotting. Oxidative stress markers, 8-OHdG, 4-HNE, and hexonoyl lesion (HEL), and other aging markers, p16 and p38, were used to assess the samples. The infiltrating mononuclear cells and endothelia of capillaries in the cGVHD and aged mice expressed the oxidative stress markers and other aging markers, but not in the young mice. Histological changes and the expression of aging markers in the samples from cGVHD mice exhibited similar features to those in aging mice. These results suggest that changes that typically appear with advanced age occur earlier in the lives of mice with lacrimal gland cGVHD.
AB - Aging is commonly defined as the accumulation of diverse deleterious changes in cells and tissues with advancing age. To investigate whether aging changes are involved in the lacrimal glands of chronic graft-versus-host disease (cGVHD) model mice, we obtained the specimens from cGVHD model mice, untreated aged and young mice, and examined by histopathology, and immunoblotting. Oxidative stress markers, 8-OHdG, 4-HNE, and hexonoyl lesion (HEL), and other aging markers, p16 and p38, were used to assess the samples. The infiltrating mononuclear cells and endothelia of capillaries in the cGVHD and aged mice expressed the oxidative stress markers and other aging markers, but not in the young mice. Histological changes and the expression of aging markers in the samples from cGVHD mice exhibited similar features to those in aging mice. These results suggest that changes that typically appear with advanced age occur earlier in the lives of mice with lacrimal gland cGVHD.
UR - https://www.scopus.com/pages/publications/84902939425
UR - https://www.scopus.com/pages/publications/84902939425#tab=citedBy
U2 - 10.1038/srep02455
DO - 10.1038/srep02455
M3 - Article
C2 - 23986066
AN - SCOPUS:84902939425
SN - 2045-2322
VL - 3
JO - Scientific reports
JF - Scientific reports
M1 - 2455
ER -