Expression cloning and intracellular localization of a human ZF5 homologue

Kazumitsu Sugiura, Yoshinao Muro, Yasuo Nagai, Takahiro Kamimoto, Takashi Wakabayashi, Masaru Ohashi, Masatoshi Hagiwara

研究成果: Article査読

18 被引用数 (Scopus)

抄録

We isolated a cDNA encoding a human homologue of ZF5 (hZF5), which has five Kruppel-like C2H2 type zinc fingers at carboxyl terminus and the BTB/POZ (poxvirus and zinc finger) at the amino terminus, using autoimmune sera from a patient with overlap syndrome (dermatomyositis and scleroderma). Sequencing of the entire cDNA revealed an open reading frame (ORF) of 1349 bp with a deduced protein sequence of 449 amino acid residues and a calculated molecular weight of 51.3 kDa. The deduced amino acid sequence of hZF5 is highly homologous to mouse ZF5 (99.3% identity). Immunofluorescence studies revealed that HA-tagged hZF5 transiently expressed in COS-7 cells showed the nuclear dot pattern in the BTB/POZ domain-dependent manner.

本文言語English
ページ(範囲)23-26
ページ数4
ジャーナルBiochimica et Biophysica Acta - Gene Structure and Expression
1352
1
DOI
出版ステータスPublished - 02-05-1997
外部発表はい

All Science Journal Classification (ASJC) codes

  • 構造生物学
  • 生物理学
  • 生化学
  • 遺伝学

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