Expression cloning of oligomerization-activated genes with cell-proliferating potency by pseudotype retrovirus vector

Akihiro Abe, Nobuhiko Emi, Tadaharu Kanie, Shizuka Imagama, Yoshie Kuno, Masahide Takahashi, Hidehiko Saito, Tomoki Naoe

研究成果: ジャーナルへの寄稿学術論文査読

15 被引用数 (Scopus)

抄録

We developed a method of clone proliferation promoting fusion genes whose proteins were activated by protein oligomerization through the helix-loop-helix region (PNT domain) of TEL. We inserted a cDNA library downstream of the PNT domain with a retrovirus vector. The resulting retrovirus infected cytokine-dependent 32D cells and cells with cytokine-independent growth were analyzed for the inserted cDNA. We cloned 25 independent fusion genes including seven kinds of partner genes. Six of the seven were a fusion of TEL with protein tyrosine kinase, LYN, HCK, FGR, SYK, FLT3, and TYK2. A serine/threonine kinase, ARAF1, was also found to fuse with TEL. These kinase fusion proteins included kinase domains with proper reading frames. These fusions may be a useful model for clarifying the downstream signal transduction of constitutive active kinase and this expression cloning method may provide a new tool with which to study cell proliferation signalling.

本文言語英語
ページ(範囲)920-926
ページ数7
ジャーナルBiochemical and Biophysical Research Communications
320
3
DOI
出版ステータス出版済み - 30-07-2004
外部発表はい

All Science Journal Classification (ASJC) codes

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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