Expression of the tyrosinase-encoding gene in a colorless melanophore mutant of the medaka fish, Oryzias latipes

Inagaki Hidehito, Bessho Yoshitaka, Koga Akihiko, Hori Hiroshi

研究成果: Article

48 引用 (Scopus)

抄録

In the medaka fish Oryzias latipes many mutants for body colors have been isolated. Among them, a colorless melanophore mutant b, carrying b alleles homozygously, has pigmented black eyes but orange-colored skin with amelanotic melanophores, suggesting the presence of a tissue-specific mechanism of melanin formation. To cast light on the molecular basis of the mechanism, we have cloned cDNAs for tyrosinase (Tyr), a key enzyme in melanin biosynthesis, from the wild-type (wt) fish. DNA sequence analysis revealed that all clones encode a protein of 540 amino acids, having five potential glycosylation sites and two copper-binding sites that are characteristic features of Tyr. Genomic DNA blot analysis disclosed that the Tyr gene is present as a single copy in the fish genome. Using a cDNA clone as a probe, RNA blot analysis was carried out. In the wt, the 2.2-kb Tyr mRNA was expressed in eyes and skin but not in liver, corresponding to tissue-specific melanin formation. In the b mutant, contrary to expectation, the mRNA was detected not only in eyes but also in amelanotic skin. Therefore, pigmentation of the skin controlled by b is not directly related to expression of the Tyr gene.

元の言語English
ページ(範囲)319-324
ページ数6
ジャーナルGene
150
発行部数2
DOI
出版物ステータスPublished - 01-01-1994
外部発表Yes

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Melanophores
Oryzias
Monophenol Monooxygenase
Fishes
Melanins
Genes
Skin
Complementary DNA
Clone Cells
Skin Pigmentation
RNA Probes
Messenger RNA
DNA Sequence Analysis
Glycosylation
Copper
Color
Alleles
Binding Sites
Genome
Gene Expression

All Science Journal Classification (ASJC) codes

  • Genetics

これを引用

Hidehito, Inagaki ; Yoshitaka, Bessho ; Akihiko, Koga ; Hiroshi, Hori. / Expression of the tyrosinase-encoding gene in a colorless melanophore mutant of the medaka fish, Oryzias latipes. :: Gene. 1994 ; 巻 150, 番号 2. pp. 319-324.
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abstract = "In the medaka fish Oryzias latipes many mutants for body colors have been isolated. Among them, a colorless melanophore mutant b, carrying b alleles homozygously, has pigmented black eyes but orange-colored skin with amelanotic melanophores, suggesting the presence of a tissue-specific mechanism of melanin formation. To cast light on the molecular basis of the mechanism, we have cloned cDNAs for tyrosinase (Tyr), a key enzyme in melanin biosynthesis, from the wild-type (wt) fish. DNA sequence analysis revealed that all clones encode a protein of 540 amino acids, having five potential glycosylation sites and two copper-binding sites that are characteristic features of Tyr. Genomic DNA blot analysis disclosed that the Tyr gene is present as a single copy in the fish genome. Using a cDNA clone as a probe, RNA blot analysis was carried out. In the wt, the 2.2-kb Tyr mRNA was expressed in eyes and skin but not in liver, corresponding to tissue-specific melanin formation. In the b mutant, contrary to expectation, the mRNA was detected not only in eyes but also in amelanotic skin. Therefore, pigmentation of the skin controlled by b is not directly related to expression of the Tyr gene.",
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Expression of the tyrosinase-encoding gene in a colorless melanophore mutant of the medaka fish, Oryzias latipes. / Hidehito, Inagaki; Yoshitaka, Bessho; Akihiko, Koga; Hiroshi, Hori.

:: Gene, 巻 150, 番号 2, 01.01.1994, p. 319-324.

研究成果: Article

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