TY - JOUR
T1 - Extensively drug-resistant IMP-16-producing Pseudomonas monteilii isolated from cerebrospinal fluid
AU - Ballaben, Anelise Stella
AU - Galetti, Renata
AU - Andrade, Leonardo Neves
AU - Ferreira, Joseane Cristina
AU - de Oliveira Garcia, Doroti
AU - Doi, Yohei
AU - Darini, Ana Lucia Costa
N1 - Funding Information:
This work was supported by FAPESP [grant 2014/14494–8 ]. Y.D. was supported by research grants from the National Institutes of Health ( R21AI135522 , R01AI104895 ). ASB was supported by a PhD fellowship [grant 2015/23484–9 ]. RG was supported by a postdoctoral fellowship from FAPESP [grant 2015/11728–0 ]. Also, this study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil ( CAPES ) - Finance Code 001.
Funding Information:
We thank to São Paulo Research Foundation (FAPESP) and National Council for Scientific and Technological Development (CNPq) for the constant support for our research. Dr. Vaughn Cooper for his assistance with whole genome sequencing. Profª. Drª. Marcia Regina von Zeska Kress and Mario Henrique Paziani for the provision of Galleria mellonella experiments.
Publisher Copyright:
© 2020
PY - 2021/1
Y1 - 2021/1
N2 - IMP-1-producing Pseudomonas aeruginosa was first reported in Japan and since then, bacteria with this metallo-β-lactamase have been detected worldwide. Pseudomonas monteilii (part of P. putida group) were considered an environmental pathogen with low virulence potential; however, multidrug-resistant and carbapenem-resistant P. monteilii have emerged. The present study reports the draft sequence of an extensively drug-resistant IMP-16-producing P. monteilii 597/14 isolated from cerebrospinal fluid in 2014. The sequencing data revealed blaIMP-16 as a gene cassette on class 1 integron, In1738 characterized in this study. Furthermore, the resistome of Pm597/14 consisted of 7 resistance genes (aadA1b, strA, strB, aacA4, blaIMP-16, blaOXA-2, sul1) and diverse virulence determinants involved in the adherence, LPS, antiphagocytosis, iron uptake and mercuric resistance. Although different virulence determinants were found in this study, using Galleria mellonella infection model, Pm597/14 did not kill any larvae between 7 days post-infection. P. monteilii isolates have been reported from clinical and environmental sources, carrying different MBL genes showing its potential role as their reservoir.
AB - IMP-1-producing Pseudomonas aeruginosa was first reported in Japan and since then, bacteria with this metallo-β-lactamase have been detected worldwide. Pseudomonas monteilii (part of P. putida group) were considered an environmental pathogen with low virulence potential; however, multidrug-resistant and carbapenem-resistant P. monteilii have emerged. The present study reports the draft sequence of an extensively drug-resistant IMP-16-producing P. monteilii 597/14 isolated from cerebrospinal fluid in 2014. The sequencing data revealed blaIMP-16 as a gene cassette on class 1 integron, In1738 characterized in this study. Furthermore, the resistome of Pm597/14 consisted of 7 resistance genes (aadA1b, strA, strB, aacA4, blaIMP-16, blaOXA-2, sul1) and diverse virulence determinants involved in the adherence, LPS, antiphagocytosis, iron uptake and mercuric resistance. Although different virulence determinants were found in this study, using Galleria mellonella infection model, Pm597/14 did not kill any larvae between 7 days post-infection. P. monteilii isolates have been reported from clinical and environmental sources, carrying different MBL genes showing its potential role as their reservoir.
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U2 - 10.1016/j.meegid.2020.104658
DO - 10.1016/j.meegid.2020.104658
M3 - Article
C2 - 33271337
AN - SCOPUS:85097737360
VL - 87
JO - Infection, Genetics and Evolution
JF - Infection, Genetics and Evolution
SN - 1567-1348
M1 - 104658
ER -