Extracellular matrix components prevent lipopolysaccharide-induced bovine arterial endothelial cell Injury by inhibiting p38 mitogen-activated protein kinase

Dipshikha Chakravortty, Yutaka Kato, Naoki Koide, Tsuyoshi Sugiyama, Makoto Kawai, Masako Fukada, Tomoaki Yoshida, Takashi Yokochi

研究成果: Article査読

5 被引用数 (Scopus)

抄録

The effect of extracellular matrix components on lipopolysaccharide-induced vascular endothelial cell injury was studied by using lipopolysaccharide-susceptible bovine aortic endothelial cells. For evaluation of lipopolysaccharide-induced injury, we estimated DNA synthesis and cell detachment of bovine aortic endothelial cells in cultures using extracellular matrix components-coated plastic dishes. Among extracellular matrix components, matrigel almost completely inhibited the reduction in DNA synthesis and the enhancement in cell detachment of bovine aortic endothelial cells in cultures with lipopolysaccharide. The lipopolysaccharide-induced injury was also inhibited by coating with type IV collagen, gelatin, fibronectin, laminin, vitronectin, and heparin sulphate proteoglycan. Extracellular matrix components capable of preventing lipopolysaccharide-induced bovine aortic endothelial cells injury coincidentally inhibited the phosphorylation of p38 mitogen-activated protein kinase in lipopolysaccharide-treated bovine aortic endothelial cells. SB203580, a specific inhibitor of p38 mitogen-activated protein kinase, also prevented the reduction in DNA synthesis and the enhancement in cell detachment of bovine aortic endothelial cells in cultures with lipopolysaccharide. It was therefore suggested that extracellular matrix components might protect bovine aortic endothelial cells from lipopolysaccharide-induced injury through inhibiting the activation of p38 mitogen-activated protein kinase.

本文言語English
ページ(範囲)187-193
ページ数7
ジャーナルThrombosis Research
98
2
DOI
出版ステータスPublished - 15-04-2000
外部発表はい

All Science Journal Classification (ASJC) codes

  • 血液学

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