Firefly luciferase as the reporter for transcriptional response to the environment in Escherichia coli

Masashi Ryo, Yuta Oshikoshi, Shosei Doi, Shogo Motoki, Atsuko Niimi, Setsuyuki Aoki

研究成果: Article査読

1 被引用数 (Scopus)

抄録

We demonstrate that firefly luciferase is a good reporter in Escherichia coli for transcription dynamics in response to the environment. E. coli strains, carrying a fusion of the promoter of the ycgZ gene and the coding region of the luciferase gene, showed transient bioluminescence on receiving blue light. This response was compromised in mutants lacking known regulators in manners consistent with each regulator's function. We also show that relA, a gene encoding a (p)ppGpp synthetase, affects ycgZ dynamics when nullified. Moreover, two unstable luciferase variants showed improved response dynamics and should be useful to study quick changes of gene expression.

本文言語English
ページ(範囲)211-213
ページ数3
ジャーナルAnalytical Biochemistry
443
2
DOI
出版ステータスPublished - 01-01-2013
外部発表はい

All Science Journal Classification (ASJC) codes

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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