First-phase validation of the in vitro eye irritation tests for cosmetic ingredients

Y. Ohno, T. Kaneko, T. Kobayashi, T. Inoue, Y. Kuroiwa, T. Yoshida, J. Momma, M. Hayashi, J. Akiyama, T. Atsumi, K. Chiba, T. Endo, A. Fujii, H. Kakishima, H. Kojima, K. Masamoto, M. Masuda, S. Matsukawa, K. Ohkoshi

研究成果: Article査読

24 被引用数 (Scopus)


Twelve alternative methods to Draize eye irritation tests were preliminarily validated using 9 coded surfactants and physiological saline solution by 20 laboratories including a national institute, universities, kit suppliers, and cosmetics companies. The results indicated that intra- and interlaboratory variances were small as a whole except for the case of hemolysis by Tween 20; rank order of the cytotoxicity potentials of Triton X-100 among samples by cultured cell methods with serum differed greatly from those without serum; correlation coefficient between in vitro methods and in vivo Draize scores is higher than 0.8 in HET-CAM-trypan blue staining method, and cultured cell methods with serum and rank order correlation coefficients were higher than 0.9; correlation among cultured cell methods using serum was also high (r = 0.92-0.99).

ジャーナルIn Vitro Toxicology: Journal of Molecular and Cellular Toxicology
出版ステータスPublished - 01-01-1994

All Science Journal Classification (ASJC) codes

  • 毒物学


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