@inproceedings{3a66a0debbd7430aa8c000d1cbdf1077,
title = "Fluorescence signal amplification for sensitive enzyme immunoassay utilizing an immuno-wall",
abstract = "This paper reports a novel method of signal amplification based on the accumulation of enzymatic fluorescent products at a wall-like structure (immuno-wall) in a microchannel for sensitive immunoassay. We found the function of an immuno-wall as a media for the enrichment of fluorescence molecules. We combined this function with the amplification of the fluorescence signal by an enzymatic reaction to produce a synergistic effect. The performance of the device was evaluated with human C-reactive protein (CRP) as a model target. The limit of detection of CRP was 2.5 pg/mL. This value was approximately 3 orders of magnitude lower than that obtained with a fluorescent dye-labeled antibody (1.7 ng/mL).",
keywords = "Biomarker Detection, Enzyme Immunoassay, Immuno-wall Device, Signal Amplification",
author = "Keine Nishiyama and Toshihiro Kasama and Masatoshi Maeki and Akihiko Ishida and Hirofumi Tani and Yoshinobu Baba and Manabu Tokeshi",
note = "Publisher Copyright: {\textcopyright} 2019 CBMS-0001.; 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019 ; Conference date: 27-10-2019 Through 31-10-2019",
year = "2019",
language = "English",
series = "23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019",
publisher = "Chemical and Biological Microsystems Society",
pages = "689--690",
booktitle = "23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019",
}