Frequent gene amplification and overexpression of decoy receptor 3 in glioblastoma

Yasuaki Arakawa, Osamu Tachibana, Mitsuhiro Hasegawa, Tadao Miyamori, Junkoh Yamashita, Yutaka Hayashi

研究成果: Article査読

32 被引用数 (Scopus)

抄録

The decoy receptor 3 (DcR3) gene is amplified at high frequency in human lung, colon, and liver cancers. DcR3 has been demonstrated to produce a secreted member of the tumor necrosis factor receptor superfamily that negatively regulates Fas-mediated apoptosis. In this study we examined DcR3 gene amplification, DcR3 mRNA expression, and DcR3 protein expression in 46 human astrocytic brain tumors by quantitative genomic PCR, quantitative reverse transcription-PCR, and immunohistochemistry, respectively. The DcR3 gene amplification was detected in none of 6 (0%) low-grade astrocytomas, 1 of 16 (6%) anaplastic astrocytomas, and 6 of 24 (25%) glioblastomas. Six of 7 (86%) cases with gene amplification exhibited both mRNA overexpression and/or protein overexpression, suggesting that DcR3 mRNA and protein were expressed more abundantly in the cases with gene amplification. We thus concluded that high DcR3 mRNA expression and protein expression may be positively related to the gene amplification in astrocytic brain tumors, especially glioblastomas. Further, we speculated that the DcR3 gene amplification with overexpression may be responsible for malignant features in glioblastomas.

本文言語English
ページ(範囲)294-298
ページ数5
ジャーナルActa Neuropathologica
109
3
DOI
出版ステータスPublished - 01-04-2005
外部発表はい

All Science Journal Classification (ASJC) codes

  • 病理学および法医学
  • 臨床神経学
  • 細胞および分子神経科学

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