Fructose induces glucose-dependent insulinotropic polypeptide, glucagon-like peptide-1 and insulin secretion: Role of adenosine triphosphate-sensitive K⁺ channels

Adenosine triphosphate-sensitive K⁺ (K_ATP) channels play an essential role in glucose-induced insulin secretion from pancreatic β-cells. It was recently reported that the K_ATP channel is also found in the enteroendocrine K-cells and L-cells that secrete glucose-dependent insulinotropic polypeptide (GIP) and glucagon-like peptide-1 (GLP-1), respectively. In the present study, we investigated the involvement of the K_ATP channel in fructose-induced GIP, GLP-1 and insulin secretion in mice. Fructose stimulated GIP secretion, but pretreatment with diazoxide, a K_ATP channel activator, did not affect fructose-induced GIP secretion under streptozotocin-induced hyperglycemic conditions. Fructose significantly stimulated insulin secretion in Kir6.2^+/+ mice, but not in mice lacking K_ATP channels (Kir6.2^-/-), and fructose stimulated GLP-1 secretion in both Kir6.2^+/+ mice and Kir6.2^-/- mice under the normoglycemic condition. In addition, diazoxide completely blocked fructose-induced insulin secretion in Kir6.2^+/+ mice and in MIN6-K8 β-cells. These results show that fructose-induced GIP and GLP-1 secretion is K_ATP channel-independent and that fructose-induced insulin secretion is K_ATP channel-dependent. Fructose significantly stimulated insulin secretion in Kir6.2^+/+ mice but not in mice lacking K_ATP channels (Kir6.2^-/-).