TY - JOUR
T1 - Full genome-based characterization of G4P[6] rotavirus strains from diarrheic patients in Thailand
T2 - Evidence for independent porcine-to-human interspecies transmission events
AU - Tacharoenmuang, Ratana
AU - Guntapong, Ratigorn
AU - Upachai, Sompong
AU - Singchai, Phakapun
AU - Fukuda, Saori
AU - Ide, Tomihiko
AU - Hatazawa, Riona
AU - Sutthiwarakom, Karun
AU - Kongjorn, Santip
AU - Onvimala, Napa
AU - Luechakham, Tipsuda
AU - Ruchusatsawast, Kriangsak
AU - Kawamura, Yoshiki
AU - Sriwanthana, Busarawan
AU - Motomura, Kazushi
AU - Tatsumi, Masashi
AU - Takeda, Naokazu
AU - Yoshikawa, Tetsushi
AU - Murata, Takayuki
AU - Uppapong, Ballang
AU - Taniguchi, Koki
AU - Komoto, Satoshi
N1 - Funding Information:
We wish to thank the public health staff of Phen and Phrapokklao Hospitals for their great help in collecting specimens and clinical data on the subjects. This study was supported in part by AMED (19fk0108099s0301 and 20fk0108099s0302) (SK), the Thailand-Japan Research Collaboration Center on Emerging and Re-emerging Infections, RCC-ERI (NT), and the laboratory-based surveillance program for unidentified and possibly new pathogens affecting public health problems in Thailand, IEIP-EID (BS).
Publisher Copyright:
© 2021, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
PY - 2021/8
Y1 - 2021/8
N2 - The exact evolutionary patterns of human G4P[6] rotavirus strains remain to be elucidated. Such strains possess unique and strain-specific genotype constellations, raising the question of whether G4P[6] strains are primarily transmitted via independent interspecies transmission or human-to-human transmission after interspecies transmission. Two G4P[6] rotavirus strains were identified in fecal specimens from hospitalized patients with severe diarrhea in Thailand, namely, DU2014-259 (RVA/Human-wt/THA/DU2014-259/2014/G4P[6]) and PK2015-1-0001 (RVA/Human-wt/THA/PK2015-1-0001/2015/G4P[6]). Here, we analyzed the full genomes of the two human G4P[6] strains, which provided the opportunity to study and confirm their evolutionary origin. On whole genome analysis, both strains exhibited a unique Wa-like genotype constellation of G4-P[6]-I1-R1-C1-M1-A8-N1-T1-E1-H1. The NSP1 genotype A8 is commonly found in porcine rotavirus strains. Furthermore, on phylogenetic analysis, each of the 11 genes of strains DU2014-259 and PK2015-1-0001 appeared to be of porcine origin. On the other hand, the two study strains consistently formed distinct clusters for nine of the 11 gene segments (VP4, VP6, VP1-VP3, and NSP2-NSP5), strongly indicating the occurrence of independent porcine-to-human interspecies transmission events. Our observations provide important insights into the origin of zoonotic G4P[6] strains, and into the dynamic interaction between porcine and human rotavirus strains.
AB - The exact evolutionary patterns of human G4P[6] rotavirus strains remain to be elucidated. Such strains possess unique and strain-specific genotype constellations, raising the question of whether G4P[6] strains are primarily transmitted via independent interspecies transmission or human-to-human transmission after interspecies transmission. Two G4P[6] rotavirus strains were identified in fecal specimens from hospitalized patients with severe diarrhea in Thailand, namely, DU2014-259 (RVA/Human-wt/THA/DU2014-259/2014/G4P[6]) and PK2015-1-0001 (RVA/Human-wt/THA/PK2015-1-0001/2015/G4P[6]). Here, we analyzed the full genomes of the two human G4P[6] strains, which provided the opportunity to study and confirm their evolutionary origin. On whole genome analysis, both strains exhibited a unique Wa-like genotype constellation of G4-P[6]-I1-R1-C1-M1-A8-N1-T1-E1-H1. The NSP1 genotype A8 is commonly found in porcine rotavirus strains. Furthermore, on phylogenetic analysis, each of the 11 genes of strains DU2014-259 and PK2015-1-0001 appeared to be of porcine origin. On the other hand, the two study strains consistently formed distinct clusters for nine of the 11 gene segments (VP4, VP6, VP1-VP3, and NSP2-NSP5), strongly indicating the occurrence of independent porcine-to-human interspecies transmission events. Our observations provide important insights into the origin of zoonotic G4P[6] strains, and into the dynamic interaction between porcine and human rotavirus strains.
UR - http://www.scopus.com/inward/record.url?scp=85107499067&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85107499067&partnerID=8YFLogxK
U2 - 10.1007/s11262-021-01851-y
DO - 10.1007/s11262-021-01851-y
M3 - Article
C2 - 34106412
AN - SCOPUS:85107499067
VL - 57
SP - 338
EP - 357
JO - Virus Genes
JF - Virus Genes
SN - 0920-8569
IS - 4
ER -